羟乙基淀粉在内皮细胞的蓄积及其对白细胞-内皮细胞相互作用的功能影响。

Endothelial accumulation of hydroxyethyl starch and functional consequences on leukocyte-endothelial interactions.

作者信息

Nohé B, Burchard M, Zanke C, Eichner M, Krump-Konvalinkova V, Kirkpatrick C J, Dieterich H-J

机构信息

Department of Anaesthesiology and Critical Care, University Hospital Tübingen, Germany.

出版信息

Eur Surg Res. 2002 Sep-Oct;34(5):364-72. doi: 10.1159/000064005.

DOI:10.1159/000064005

PMID:12364821

Abstract

To date, accumulation of hydroxyethyl starch (HES) has been studied mainly in skin specimens, but there are no detailed reports available regarding starch accumulation in the endothelium. Because endothelial cells play an essential role during shock, we studied the accumulation of HES in human umbilical venous endothelial cells (HUVEC). HUVEC (n = 9) were incubated with a fluorescein-conjugated HES 200/0.5 (FITC-HES) at 0.5-20 mg/ml for 1-72 h. FITC-HES was internalized dose- and time-dependently by pinocytosis into secondary lysosomes. Asymptotic elimination curves showed that 50% of the formerly ingested molecules could not be eliminated. Despite accumulation, starch molecules did not attenuate the expression of E-selectin, ICAM-1 or VCAM-1 on TNF-alpha-activated HUVEC. However, apart from adhesion molecule expression, perfusion studies showed that HES reduced neutrophil adhesion by direct inhibition of integrin-mediated interactions.

摘要

迄今为止，羟乙基淀粉（HES）的蓄积主要在皮肤标本中进行了研究，但关于淀粉在内皮中的蓄积尚无详细报道。由于内皮细胞在休克过程中发挥着重要作用，我们研究了HES在人脐静脉内皮细胞（HUVEC）中的蓄积情况。将HUVEC（n = 9）与荧光素标记的HES 200/0.5（FITC-HES）以0.5 - 20 mg/ml的浓度孵育1 - 72小时。FITC-HES通过胞饮作用以剂量和时间依赖性方式内化到次级溶酶体中。渐近消除曲线显示，先前摄取的分子中有50%无法被消除。尽管有蓄积，但淀粉分子并未减弱TNF-α激活的HUVEC上E-选择素、ICAM-1或VCAM-1的表达。然而，除了黏附分子表达外，灌注研究表明，HES通过直接抑制整合素介导的相互作用减少了中性粒细胞的黏附。