Collis R E, Collins P W, Gutteridge C N, Kaul A, Newland A C, Williams D M, Webb A R
Bloomsbury Department of Intensive Care, Middlesex Hospital, London, UK.
Intensive Care Med. 1994;20(1):37-41. doi: 10.1007/BF02425053.
To study the effect of medium molecular weight hydroxyethyl starches on endothelial cell and neutrophil activation in vitro.
Laboratory analysis.
The effects of albumin and hydroxyethyl starch on the neutrophil adhesion molecule (CD11bCD18), with and without lipopolysaccharide stimulation, were studied in whole blood. E-selectin expression on human umbilical vein endothelial cells was stimulated with lipopolysaccharide alone and in the presence of either albumin or hydroxyethyl starch. The effect of albumin and hydroxyethyl starches on rapid endothelial cell activation was studied using von Willebrand factor release as a marker.
Hydroxyethyl starches but not albumin inhibited stimulated vWF release in a dose dependent manner. No effect was seen on endothelial E-selectin or neutrophil CD11bCD18 expression.
these results suggest a possible beneficial role of hydroxyethyl starches in the inhibition of endothelial activation thus preventing neutrophil adhesion during sepsis syndrome.
研究中分子量羟乙基淀粉对体外内皮细胞和中性粒细胞活化的影响。
实验室分析。
在全血中研究白蛋白和羟乙基淀粉对中性粒细胞黏附分子（CD11bCD18）的影响，有无脂多糖刺激。单独用脂多糖以及在有白蛋白或羟乙基淀粉存在的情况下刺激人脐静脉内皮细胞上E-选择素的表达。以血管性血友病因子释放为标志物，研究白蛋白和羟乙基淀粉对快速内皮细胞活化的影响。
羟乙基淀粉而非白蛋白以剂量依赖性方式抑制刺激后的vWF释放。对内皮E-选择素或中性粒细胞CD11bCD18表达未见影响。
这些结果提示羟乙基淀粉在抑制内皮细胞活化从而预防脓毒症综合征期间中性粒细胞黏附方面可能具有有益作用。
