Byrne M F, Corcoran P A, Atherton J C, Sheehan K M, Murray F E, Fitzgerald D J, Murphy J F
Department of Clinical Pharmacology, Royal College of Surgeons in Ireland, Dublin, Ireland.
FEBS Lett. 2002 Dec 18;532(3):411-4. doi: 10.1016/s0014-5793(02)03728-6.
Helicobacter pylori upregulates endothelial adhesion molecules but the pattern is unclear. Human umbilical vein endothelial cells (HUVEC) were exposed to control medium or H. pylori 60190. Binding of monoclonal antibodies against P-selectin, E-selectin, vascular adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) was determined using enzyme-linked immunosorbent assay. Binding of polymorphonuclear leukocytes to HUVEC was determined on cells exposed as above. After 6 h exposure to H. pylori, there were 30%, 124%, 167% and 100% increases in P-selectin, E-selectin, VCAM-1 and ICAM-1 levels and a 400% increase in polymorphonuclear leukocyte adhesion in HUVEC exposed to H. pylori. Effects of incubation for other intervals between 0 and 18 h are also described. H. pylori exerts some of its effects on gastric mucosa via gastric vasculature. This study gives insight into the pattern of H. pylori-associated endothelial adhesion molecule upregulation.
幽门螺杆菌上调内皮黏附分子，但其模式尚不清楚。将人脐静脉内皮细胞（HUVEC）暴露于对照培养基或幽门螺杆菌60190中。使用酶联免疫吸附测定法测定抗P-选择素、E-选择素、血管细胞黏附分子-1（VCAM-1）和细胞间黏附分子-1（ICAM-1）的单克隆抗体的结合情况。在上述暴露的细胞上测定多形核白细胞与HUVEC的结合情况。在暴露于幽门螺杆菌6小时后，暴露于幽门螺杆菌的HUVEC中P-选择素、E-选择素、VCAM-1和ICAM-1水平分别增加了30%、124%、167%和100%，多形核白细胞黏附增加了400%。还描述了在0至18小时之间其他孵育时间间隔的影响。幽门螺杆菌通过胃血管系统对胃黏膜发挥一些作用。这项研究深入了解了幽门螺杆菌相关内皮黏附分子上调的模式。
