Smith R F
Health Lab Sci. 1975 Jul;12(3):219-24.
Corynebacterium vaginale was isolated from 48 (23.4%) cervical, vaginal, and urethral swabbings of 205 female outpatients. A new medium modified from peptone starch dextrose agar was used to isolate C. vaginale from most specimens. The new medium contained starch without dextrose with brom cresol purple added. With the exception of certain strains of streptococci, C. vaginale was the only organism isolated from genital specimens that grew and fermented starch on the medium. The new medium provided a rapid means of detecting and isolating C. vaginale compared to other media, and for estimating the relative predominance of the organism in mixed culture. Gram strains were used to detect clue cells in direct smears of clinical material. These cells were detected in only 12 of 48 specimens from which C. vaginale was isolated. Smears on 8 additional specimens contained clue cells but C. vaginale was not isolated. Purple broth base was superior to cystine trypticase agar for confirmatory tests with C. vaginale.
从205名女性门诊患者的48份（23.4%）宫颈、阴道和尿道拭子中分离出阴道棒状杆菌。一种从蛋白胨淀粉葡萄糖琼脂改良而来的新培养基被用于从大多数标本中分离阴道棒状杆菌。这种新培养基含有不加葡萄糖的淀粉，并添加了溴甲酚紫。除了某些链球菌菌株外，阴道棒状杆菌是从生殖器标本中分离出的唯一能在该培养基上生长并发酵淀粉的微生物。与其他培养基相比，这种新培养基提供了一种快速检测和分离阴道棒状杆菌的方法，以及估计该微生物在混合培养物中相对优势的方法。革兰氏染色用于检测临床材料直接涂片上的线索细胞。在分离出阴道棒状杆菌的48份标本中，仅在12份中检测到这些细胞。另外8份标本的涂片含有线索细胞，但未分离出阴道棒状杆菌。紫色肉汤基础培养基在阴道棒状杆菌的确认试验中优于胱氨酸胰蛋白酶琼脂。
