通过直接免疫荧光法检测阴道标本中的阴道加德纳菌。
Detection of Gardnerella vaginalis in vaginal specimens by direct immunofluorescence.
作者信息
Hansen W, Vray B, Miller K, Crokaert F, Yourassowsky E
机构信息
Department of Microbiology, Brugmann University Hospital, Brussels, Belgium.
出版信息
J Clin Microbiol. 1987 Oct;25(10):1934-7. doi: 10.1128/jcm.25.10.1934-1937.1987.
The preparation of a fluorescein-labeled Gardnerella vaginalis polyclonal antibody is described, and its usefulness is assessed for the detection of this microorganism in vaginal samples obtained from 263 women attending the gynecological department of a general hospital, 66 of whom harbored an intrauterine device. The direct immunofluorescence technique was positive for G. vaginalis in 21% of the specimens, whereas only 12.5% of the total bacteriological cultures were positive. The frequency was higher in patients harboring intrauterine devices since 34.8% exhibited positive immunofluorescence and 30.3% exhibited positive cultures.
本文描述了荧光素标记的阴道加德纳菌多克隆抗体的制备，并评估了其在检测某综合医院妇科就诊的263名女性阴道样本中该微生物的效用，其中66名女性带有宫内节育器。直接免疫荧光技术检测显示，21%的标本中阴道加德纳菌呈阳性，而细菌培养总体阳性率仅为12.5%。带有宫内节育器的患者中该菌检出频率更高，因为34.8%的患者免疫荧光呈阳性，30.3%的患者培养结果呈阳性。
Zotero 插件