Gomez María R, Olsina Roberto A, Martínez Luis D, Silva María F
Cátedra de Control de Calidad de Medicamentos, Universidad Nacional de San Luis, Facultad de Química, Bioquímica y Farmacia, Chacabuco y Pedernera, San Luis 5700, Argentina.
J Pharm Biomed Anal. 2002 Oct 15;30(3):791-9. doi: 10.1016/s0731-7085(02)00362-x.
The separation of basic nitrogenous compounds commonly used as active ingredients in cold medicine formulations by micellar electrokinetic capillary chromatography and capillary zone electrophoresis with direct absorptiometric detection was investigated. The type and composition of the background electrolyte (BGE) were investigated with respect to separation selectivity and BGE stability. BGE of 10 mM sodium dihydrogenphosphate-sodium tetraborate buffer containing 10 mM SDS and 10% acetonitrile, pH 9.0 was found to be optimal. Dextromethorphan hydrobhromide, diphenhydramine hydrochloride and phenylephrine hydrochloride were baseline-separated in less than 11 min, giving separation efficiencies of up to 494,000 theoretical plates, reproducibility of corrected peaks areas below 3% relative standard deviation and concentration detection limits from 2.5 to 5.5 microg ml(-1). Detection was performed at 196 and 214 nm.
研究了采用胶束电动毛细管色谱法和毛细管区带电泳法并结合直接吸光检测法对感冒药制剂中常用作活性成分的碱性含氮化合物进行分离。考察了背景电解质（BGE）的类型和组成对分离选择性和BGE稳定性的影响。发现含10 mM十二烷基硫酸钠（SDS）和10%乙腈的10 mM磷酸二氢钠-硼砂缓冲液（pH 9.0）作为BGE最为合适。氢溴酸右美沙芬、盐酸苯海拉明和盐酸去氧肾上腺素在不到11分钟内实现基线分离，分离效率高达494,000理论塔板数，校正峰面积的重现性相对标准偏差低于3%，浓度检测限为2.5至5.5 μg ml⁻¹。检测在196和214 nm波长下进行。
