Boyson Jonathan E, Rybalov Basya, Koopman Louise A, Exley Mark, Balk Steven P, Racke Frederick K, Schatz Frederick, Masch Rachel, Wilson S Brian, Strominger Jack L
Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.
Proc Natl Acad Sci U S A. 2002 Oct 15;99(21):13741-6. doi: 10.1073/pnas.162491699. Epub 2002 Oct 4.
Invariant CD1d-restricted natural killer T (iNKT) cells comprise a small, but significant, immunoregulatory T cell subset. Here, the presence of these cells and their CD1d ligand at the human maternal-fetal interface was investigated. Immunohistochemical staining of human decidua revealed the expression of CD1d on both villous and extravillous trophoblasts, the fetal cells that invade the maternal decidua. Decidual iNKT cells comprised 0.48% of the decidual CD3+ T cell population, a frequency 10 times greater than that seen in peripheral blood. Interestingly, decidual CD4+ iNKT cells exhibited a striking Th1-like bias (IFN-gamma production), whereas peripheral blood CD4+ iNKT clones exhibited a Th2-like bias (IL-4 production). Moreover, compared to their peripheral blood counterparts, decidual iNKT clones were strongly polarized toward granulocyte/macrophage colony-stimulating factor production. The demonstration of CD1d expression on fetal trophoblasts together with the differential pattern of cytokine expression by decidual iNKT cells suggests that maternal iNKT cell interactions with CD1d expressed on invading fetal cells may play an immunoregulatory role at the maternal-fetal interface.
不变自然杀伤T（iNKT）细胞是一种数量虽少但十分重要的免疫调节性T细胞亚群。在此，我们研究了这些细胞及其CD1d配体在人类母胎界面的存在情况。对人蜕膜进行免疫组织化学染色显示，绒毛和侵入母体蜕膜的胎儿细胞——绒毛外滋养层细胞均表达CD1d。蜕膜iNKT细胞占蜕膜CD3 + T细胞群体的0.48%，这一频率比外周血中的频率高10倍。有趣的是，蜕膜CD4 + iNKT细胞表现出明显的Th1样偏向（产生干扰素-γ），而外周血CD4 + iNKT克隆则表现出Th2样偏向（产生白细胞介素-4）。此外，与外周血中的iNKT克隆相比，蜕膜iNKT克隆强烈偏向于产生粒细胞/巨噬细胞集落刺激因子。胎儿滋养层细胞上CD1d的表达以及蜕膜iNKT细胞细胞因子表达的差异模式表明，母体iNKT细胞与侵入胎儿细胞上表达的CD1d之间的相互作用可能在母胎界面发挥免疫调节作用。
