Pérez Cristian A, Huang Liquan, Rong Minqing, Kozak J Ashot, Preuss Axel K, Zhang Hailin, Max Marianna, Margolskee Robert F
Howard Hughes Medical Institute, Mount Sinai School of Medicine, New York University, Box 1677, 1425 Madison Avenue, New York, New York 10029, USA.
Nat Neurosci. 2002 Nov;5(11):1169-76. doi: 10.1038/nn952.
We used differential screening of cDNAs from individual taste receptor cells to identify candidate taste transduction elements in mice. Among the differentially expressed clones, one encoded Trpm5, a member of the mammalian family of transient receptor potential (TRP) channels. We found Trpm5 to be expressed in a restricted manner, with particularly high levels in taste tissue. In taste cells, Trpm5 was coexpressed with taste-signaling molecules such as alpha-gustducin, Ggamma13, phospholipase C-beta2 (PLC-beta2) and inositol 1,4,5-trisphosphate receptor type III (IP3R3). Our heterologous expression studies of Trpm5 indicate that it functions as a cationic channel that is gated when internal calcium stores are depleted. Trpm5 may be responsible for capacitative calcium entry in taste receptor cells that respond to bitter and/or sweet compounds.
我们利用对来自单个味觉受体细胞的cDNA进行差异筛选，来鉴定小鼠中潜在的味觉转导元件。在差异表达的克隆中，有一个编码Trpm5，它是哺乳动物瞬时受体电位（TRP）通道家族的成员。我们发现Trpm5以一种受限的方式表达，在味觉组织中的表达水平特别高。在味觉细胞中，Trpm5与味觉信号分子如α - 味导素、Gγ13、磷脂酶C - β2（PLC - β2）和III型肌醇1,4,5 - 三磷酸受体（IP3R3）共表达。我们对Trpm5的异源表达研究表明，它作为一个阳离子通道发挥作用，当细胞内钙库耗尽时被门控。Trpm5可能负责对苦味和/或甜味化合物作出反应的味觉受体细胞中的钙释放激活钙内流。
