Turon F, Bachain P, Caro Y, Pina M, Graille J
Sea Oil, Beaumont-Hague, France.
Lipids. 2002 Aug;37(8):817-21. doi: 10.1007/s11745-002-0966-3.
An analytical procedure was developed for regiodistribution analysis of TAG using alpha-MAG prepared by an ethyl magnesium bromide deacylation. In the present communication, the deacylation procedure is shown to lead to representative alpha-MAG, allowing the composition of the native TAG in the alpha-position to be determined directly. The composition in the beta-position can then be estimated from the composition of the alpha-MAG and TAG according to the formula 3 x TAG - 2 x alpha-MAG. The estimates are superior to those obtained using the alpha,beta-DAG and Brockerhoff calculations as they come closer to the theoretical value and have smaller SD. The present procedure, first demonstrated on a synthetic TAG, was then successfully applied to the analysis of borage oil, milkfat, and tuna oil.
开发了一种分析程序，用于使用通过乙基溴化镁脱酰基制备的α-MAG对TAG进行区域分布分析。在本通讯中，脱酰基程序显示可产生具有代表性的α-MAG，从而能够直接测定天然TAG在α位的组成。然后可以根据公式3×TAG - 2×α-MAG，从α-MAG和TAG的组成估算β位的组成。这些估算值优于使用α,β-DAG和布罗克霍夫计算获得的估算值，因为它们更接近理论值且标准差更小。本程序首先在合成TAG上得到验证，随后成功应用于琉璃苣油、乳脂和金枪鱼油的分析。
