Leyk W, Scheffler R
Zentralbl Bakteriol Orig A. 1975 May;231(4):519-24.
Multiplication and content of Coxiella burneti after infection of the yolk sac were studied in different tissues and organs of embryonated hen's eggs (yolk sac, CAM, allantoamnion, amniotic fluid, liver, muscles, spleen, and heart). Demonstration of the agent was performed from the 6th to the 11th day p.i. by direct microscopic methods (Stamp and auramine staining, fluorescent antibody technique); the Coxiella content was determined by titration in embryonated hen's eggs. Coxiellae could be demonstrated, apart from the yolk sac membrane, also in the intestine and CAM from the 7th day p.i., and in the other organs examined from the 11th day p.i. by means of microscopy. For the same period, embryonic death rates (ELD50) and egg infectivity (EID50) were determined separately. The highest numbers of Coxiella were consistently found in the yolk sac membrane (ELD50 10(4.0)-10(5.2); EID50 10(6.0)-10(7.8)). In the intestine, CAM, allantoamnion, and amniotic fluid, ELD50 values were between 10(2.0) and 10(2.8), EDI50 values between 10(4.8) and 10(5.2), on the 11th day p.i.. Titres were lower for muscles, liver, spleen, and heart of infected embryos.
研究了贝氏柯克斯体感染鸡胚卵黄囊后，在鸡胚不同组织和器官（卵黄囊、绒毛尿囊膜、尿囊羊膜、羊水、肝脏、肌肉、脾脏和心脏）中的增殖情况及含量。在感染后第6天至第11天，通过直接显微镜检查方法（Stamp染色和金胺染色、荧光抗体技术）检测病原体；通过在鸡胚中滴定来测定贝氏柯克斯体的含量。除卵黄囊膜外，在感染后第7天，通过显微镜检查还可在肠道和绒毛尿囊膜中检测到贝氏柯克斯体，在感染后第11天，在其他检查的器官中也可检测到。在同一时期，分别测定胚胎死亡率（ELD50）和卵感染性（EID50）。始终在卵黄囊膜中发现贝氏柯克斯体数量最多（ELD50为10(4.0)-10(5.2)；EID50为10(6.0)-10(7.8)）。在感染后第11天，肠道、绒毛尿囊膜、尿囊羊膜和羊水中的ELD50值在10(2.0)至10(2.8)之间，EDI50值在10(4.8)至10(5.2)之间。感染胚胎的肌肉、肝脏、脾脏和心脏中的滴度较低。
