Basher H A, Fowler D R, Rodgers F G, Seaman A, Woodbine M
Zentralbl Bakteriol Mikrobiol Hyg A. 1984 Apr;256(4):477-509.
The LD50 for 15-day old chicken embryos inoculated with Listeria monocytogenes into the allantoic sac was determined. The growth cycle of the organism was investigated in different tissues and fluids derived from embryonated eggs following inoculation with a suspension of L. monocytogenes equivalent to the LD50. Eggs receiving doses of 100 and 1000 times the LD50 were used to examine the effect of high doses on the pathogenesis and growth of Listeria in ovo. The pattern of growth of the organism in embryonic blood showed two distinct peaks and correlated with these was the development of large and small pock lesions on the chorioallantoic membrane. Bacterial growth in the internal organs exhibited a single peak. Histological and electron microscopic evidence indicated that the primary cellular damage was due to a soluble haemolysin present prior to the establishment of the organism within the tissues.
测定了将单核细胞增生李斯特菌接种到15日龄鸡胚尿囊腔中的半数致死剂量（LD50）。在用相当于LD50的单核细胞增生李斯特菌悬液接种后，研究了该菌在来自胚胎蛋的不同组织和液体中的生长周期。使用接受剂量为LD50的100倍和1000倍的鸡蛋来检查高剂量对卵内李斯特菌发病机制和生长的影响。该菌在胚胎血液中的生长模式显示出两个明显的峰值，与之相关的是绒毛尿囊膜上大小痘疱病变的发展。细菌在内脏器官中的生长呈现出一个峰值。组织学和电子显微镜证据表明，主要的细胞损伤是由于在该菌在组织内定植之前就存在的一种可溶性溶血素所致。
