Higa Sayomi, Maeda Noriko, Kenmochi Naoya, Tanaka Tatsuo
Department of Biochemistry, School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, 903-0215, Okinawa, Japan.
Biochem Biophys Res Commun. 2002 Oct 11;297(5):1344-9. doi: 10.1016/s0006-291x(02)02377-x.
Many nucleotides in rRNAs are modified. We devised a method to locate 2(')-O-methyl nucleotide residues using a conventional DNA sequencer. We found 38 2(')-O-methyl nucleotides in the 26S rRNA of Caenorhabditis elegans using this method. Fourteen of the 38 residues are conserved in both human and yeast rRNAs and 14 residues are conserved in either human or yeast rRNA. The remaining 10 nucleotides are uniquely methylated in C. elegans 26S rRNA. We searched the C. elegans genomic sequence for small nucleolar RNAs (snoRNAs), which guide the methylation of ribose residues, and predicted 18 snoRNA sequences that are expected to guide the methylation of some of these nucleotide residues.
核糖体RNA（rRNA）中的许多核苷酸都经过了修饰。我们设计了一种方法，利用传统的DNA测序仪来定位2'-O-甲基核苷酸残基。我们使用这种方法在秀丽隐杆线虫的26S rRNA中发现了38个2'-O-甲基核苷酸。这38个残基中有14个在人类和酵母rRNA中都保守，14个残基在人类或酵母rRNA中保守。其余10个核苷酸在秀丽隐杆线虫26S rRNA中是独特甲基化的。我们在秀丽隐杆线虫基因组序列中搜索指导核糖残基甲基化的小核仁RNA（snoRNA），并预测了18个snoRNA序列，预计它们会指导其中一些核苷酸残基的甲基化。
