Eyal-Giladi H, Kochav S, Yerushalmi S
Differentiation. 1975 Oct 16;4(2):57-60. doi: 10.1111/j.1432-0436.1975.tb01441.x.
Tritium-labelled disaggregated chick hypoblast cells were mixed with non-labelled epiblast cells and vice-versa. The mixtures were allowed to aggregate in a gyratory shaker and were transferred on to a solid culture medium for further incubation. The aggregates were fixed after various incubation times, sectioned and examined for sorting-out. There was already a tendency to sort out after 10 h of incubation, a process which was completed after 25 h. The hypoblast cells formed a continuous layer adjacent to the vitelline membrane, while the epiblast cells moved out to form the upper external layer. The position of the two layers was normal as far as the substrate and external environment are concerned, and reversed in relation to their relative position to the vitelline membrane. The hypoblast cells tended to migrate to the margins of the aggregate. The latter phenomenon seems to parallel the migration of hypoblast cells towards the extra-embryonal area during the formation of the primitive streak.
将氚标记的分散鸡下胚层细胞与未标记的上胚层细胞混合，反之亦然。混合物在回转振荡器中聚集，然后转移到固体培养基上进一步培养。在不同的培养时间后将聚集体固定、切片并检查细胞分选情况。培养10小时后就已经有细胞分选的趋势，这个过程在25小时后完成。下胚层细胞形成与卵黄膜相邻的连续层，而上胚层细胞向外移动形成上层外层。就底物和外部环境而言，两层的位置是正常的，但相对于它们与卵黄膜的相对位置则是相反的。下胚层细胞倾向于迁移到聚集体的边缘。后一种现象似乎与原条形成过程中下胚层细胞向胚外区域的迁移相似。
