Phillips John Robert, Zalik Sara E
Department of Zoology, University of Alberta, T6G 2E9, Edmonton, Alberta, Canada.
Wilehm Roux Arch Dev Biol. 1982 Jul;191(4):234-240. doi: 10.1007/BF00848410.
The lectin-mediated agglutinability of cells dissociated from different areas of the gastrulating chick embryo was investigated. Differences in agglutinability were quantified by using a Coulter counter. Cells from the area pellucida (AP) and those from the endoderm of the area opaca (AOEn) are agglutinated by Concanavalin A (Con A), wheat germ agglutinin (WGA) andRicinus communis agglutinin (RCA). In cells from both areas the greatest agglutination response is obtained with RCA. Trypsinization of AOEn cells enhances their agglutinability with Con A, WGA and RCA. The lectin-induced agglutinability of cells from the area pellucida is similar in EDTA-dissociated and trypsinized cells.Cells from the AP are significantly more agglutinable with Con A than those of the AOEn regardless whether the former are obtained by trypsinization or dissociation with EDTA. The higher agglutinability of cells of the area pellucida with Con A, as well as the differential enhancement by trypsin of the agglutinability of AOEn cells with Con A, WGA, and RCA may reflect a difference in the cell surface glycoreceptors between the cells of the are pellucida (predominantly embryonic) and the first extraembryonic (AOEn) cell line. These cells have been shown to sort out from each other at the earliest stages of development.
对从原肠胚形成期的鸡胚不同区域解离的细胞进行了凝集素介导的凝集能力研究。通过使用库尔特计数器对凝集能力的差异进行了量化。来自明区（AP）的细胞和来自暗区内胚层（AOEn）的细胞可被刀豆球蛋白A（Con A）、麦胚凝集素（WGA）和蓖麻凝集素（RCA）凝集。在这两个区域的细胞中，RCA引起的凝集反应最强。AOEn细胞经胰蛋白酶处理后，其对Con A、WGA和RCA的凝集能力增强。明区细胞在EDTA解离和胰蛋白酶处理后的细胞中，凝集素诱导的凝集能力相似。无论AP区的细胞是通过胰蛋白酶处理还是EDTA解离获得，其对Con A的凝集能力都明显高于AOEn区的细胞。明区细胞对Con A的较高凝集能力，以及胰蛋白酶对AOEn细胞与Con A、WGA和RCA凝集能力的差异增强，可能反映了明区（主要是胚胎）细胞和最早的胚外（AOEn）细胞系细胞表面糖受体的差异。这些细胞在发育的最早阶段就已被证明会彼此分离。
