Bergenholtz A, Lemperg R K
In Vitro. 1975 Sep-Oct;11(5):286-90. doi: 10.1007/BF02615639.
Articular cartilage from 5-month-old calves survived for 4 weeks in organ culture in a chemically defined medium. The viability was assessed by calculating the labeling frequency of chondrocytes on autoradiograms after [35S]sulfate labeling. The survival rate of chondrocytes was highest in 20% oxygen tension, whereas 50% oxygen resulted in chondrocyte death after 2 weeks. The survival rate was improved by using 1-mm thick standardized pieces of cartilage. The cells of the superficial layer showed a higher proportion of living chondrocytes than the cells in the deeper layers. No differentiation, dedifferentiation, or outgrowth of cells occurred during the experimental period.
5个月大的小牛的关节软骨在化学成分明确的培养基中进行器官培养时存活了4周。通过计算[35S]硫酸盐标记后放射自显影片上软骨细胞的标记频率来评估活力。软骨细胞的存活率在20%氧张力下最高，而50%氧在2周后导致软骨细胞死亡。使用1毫米厚的标准化软骨片可提高存活率。表层细胞中存活软骨细胞的比例高于深层细胞。在实验期间未发生细胞分化、去分化或细胞增殖。
