McKenzie L S, Horsburgh B A, Ghosh P, Taylor T K
In Vitro. 1977 Jul;13(7):423-8. doi: 10.1007/BF02615102.
An organ culture system is described for adult human articular cartilage obtained from joints after femoral head replacement operations. Cartilage slices maintain maximal viability for 2 days in culture as assessed by uptake of [3H]uridine and [3H]leucine into whole tissue, and 35SO4 into sulphated glycosaminoglycans (GAGs). Since GAGs are the components of cartilage matrix, the depletion of which is associated with osteoarthrosis, a method for measuring sulphated GAG synthesis in culture has been investigated.
本文描述了一种用于从股骨头置换手术后的关节中获取的成人人类关节软骨的器官培养系统。通过将[3H]尿苷和[3H]亮氨酸摄取到整个组织中以及将35SO4摄取到硫酸化糖胺聚糖（GAG）中来评估，软骨切片在培养中可维持2天的最大活力。由于GAG是软骨基质的组成成分，其消耗与骨关节炎相关，因此研究了一种在培养中测量硫酸化GAG合成的方法。
