鼠伤寒沙门氏菌中严格调控的基因表达系统。
Tightly regulated gene expression system in Salmonella enterica serovar Typhimurium.
作者信息
McKinney Jeffrey, Guerrier-Takada Cecilia, Galán Jorge, Altman Sidney
机构信息
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA.
出版信息
J Bacteriol. 2002 Nov;184(21):6056-9. doi: 10.1128/JB.184.21.6056-6059.2002.
Abstract
A new Salmonella enterica serovar Typhimurium strain has been constructed to facilitate tightly regulated gene expression. Arabinose-inducible and glucose-repressible expression of a T7 RNA polymerase gene that has been integrated with an adjacent araC-P(BAD) control element into the bacterial chromosome allows dynamic control of T7 promoter-driven RNA transcription.
摘要
构建了一种新的肠炎沙门氏菌鼠伤寒血清型菌株，以促进基因表达的严格调控。将一个与相邻的阿拉伯糖操纵子启动子（araC-P(BAD)）控制元件整合的T7 RNA聚合酶基因置于阿拉伯糖诱导和葡萄糖抑制的表达调控之下，从而可对T7启动子驱动的RNA转录进行动态控制，该基因已整合到细菌染色体中。
相似文献
1
Tightly regulated gene expression system in Salmonella enterica serovar Typhimurium.鼠伤寒沙门氏菌中严格调控的基因表达系统。
J Bacteriol. 2002 Nov;184(21):6056-9. doi: 10.1128/JB.184.21.6056-6059.2002.
2
Two AraC/XylS family members can independently counteract the effect of repressing sequences upstream of the hilA promoter.两个AraC/XylS家族成员可独立抵消hilA启动子上游抑制序列的抑制作用。
Mol Microbiol. 1999 May;32(3):629-42. doi: 10.1046/j.1365-2958.1999.01381.x.
3
A Salmonella-based, propionate-inducible, expression system for Salmonella enterica.一种基于沙门氏菌的、丙酸盐诱导型的肠炎沙门氏菌表达系统。
Gene. 2006 Aug 1;377:6-11. doi: 10.1016/j.gene.2006.02.013. Epub 2006 Apr 17.
4
RtsA and RtsB coordinately regulate expression of the invasion and flagellar genes in Salmonella enterica serovar Typhimurium.RtsA和RtsB协同调节鼠伤寒沙门氏菌中侵袭基因和鞭毛基因的表达。
J Bacteriol. 2003 Sep;185(17):5096-108. doi: 10.1128/JB.185.17.5096-5108.2003.
5
Mutations in the araC gene of Salmonella typhimurium LT2 which affect both activator and auto-regulatory functions of the AraC protein.鼠伤寒沙门氏菌LT2的araC基因突变，该突变影响AraC蛋白的激活和自调控功能。
Gene. 1992 Aug 1;117(1):31-7. doi: 10.1016/0378-1119(92)90486-9.
6
AraC protein can activate transcription from only one position and when pointed in only one direction.阿糖胞苷蛋白只能从一个位置并仅在一个方向上激活转录。
J Mol Biol. 1993 May 20;231(2):205-18. doi: 10.1006/jmbi.1993.1276.
7
Remote control of tumour-targeted Salmonella enterica serovar Typhimurium by the use of L-arabinose as inducer of bacterial gene expression in vivo.利用L-阿拉伯糖作为体内细菌基因表达的诱导剂对肿瘤靶向性鼠伤寒沙门氏菌进行远程控制。
Cell Microbiol. 2007 Jun;9(6):1529-37. doi: 10.1111/j.1462-5822.2007.00890.x. Epub 2007 Feb 9.
8
Transcription modulation of Salmonella enterica serovar Typhimurium promoters by sub-MIC levels of rifampin.鼠伤寒沙门氏菌血清型鼠伤寒亚种启动子在利福平亚抑菌浓度水平下的转录调控
J Bacteriol. 2006 Nov;188(22):7988-91. doi: 10.1128/JB.00791-06. Epub 2006 Sep 15.
9
araB Gene and nucleotide sequence of the araC gene of Erwinia carotovora.胡萝卜软腐欧文氏菌的araB基因和araC基因的核苷酸序列。
J Bacteriol. 1985 Nov;164(2):717-22. doi: 10.1128/jb.164.2.717-722.1985.
10
Mutations resulting in promoter-like sequences which enhance the expression of araC in Salmonella typhimurium.导致启动子样序列的突变，这些序列增强了鼠伤寒沙门氏菌中araC的表达。
Gene. 1986;46(1):113-21. doi: 10.1016/0378-1119(86)90173-3.
引用本文的文献
1
In Typhimurium and , Nucleoid-Associated HU Proteins Are -Terminally Acetylated.在鼠伤寒沙门氏菌中，类核相关的HU蛋白在C末端被乙酰化。
Pathogens. 2025 Jun 20;14(7):616. doi: 10.3390/pathogens14070616.
2
Small Intestinal Goblet Cells Control Humoral Immune Responses and Mobilization During Enteric Infection.小肠杯状细胞在肠道感染期间控制体液免疫反应和免疫细胞动员。
bioRxiv. 2024 Jan 7:2024.01.06.573891. doi: 10.1101/2024.01.06.573891.
3
The Cysteine Desulfurase IscS Is a Significant Target of 2-Aminoacrylate Damage in Pseudomonas aeruginosa.半胱氨酸脱硫酶 IscS 是铜绿假单胞菌中 2-氨基丙烯酸损伤的重要靶点。
mBio. 2022 Jun 28;13(3):e0107122. doi: 10.1128/mbio.01071-22. Epub 2022 Jun 2.
4
2-Aminoacrylate stress damages diverse PLP-dependent enzymes in vivo.2-氨基丙烯酸酯应激损伤体内多种依赖 PLP 的酶。
J Biol Chem. 2022 Jun;298(6):101970. doi: 10.1016/j.jbc.2022.101970. Epub 2022 Apr 20.
5
A Platform for Deep Sequence-Activity Mapping and Engineering Antimicrobial Peptides.一个用于深度序列-活性图谱分析和抗菌肽工程的平台。
ACS Synth Biol. 2021 Oct 15;10(10):2689-2704. doi: 10.1021/acssynbio.1c00314. Epub 2021 Sep 10.
6
Goblet cell associated antigen passages are inhibited during Salmonella typhimurium infection to prevent pathogen dissemination and limit responses to dietary antigens.杯状细胞相关抗原途径在感染鼠伤寒沙门氏菌时受到抑制，以防止病原体传播并限制对膳食抗原的反应。
Mucosal Immunol. 2018 Jul;11(4):1103-1113. doi: 10.1038/s41385-018-0007-6. Epub 2018 Feb 14.
7
CCR6 promotes steady-state mononuclear phagocyte association with the intestinal epithelium, imprinting and immune surveillance.CCR6促进稳态下单核吞噬细胞与肠上皮的结合、印记形成及免疫监视。
Immunology. 2017 Dec;152(4):613-627. doi: 10.1111/imm.12801. Epub 2017 Aug 30.
8
Mutational analysis of the Shigella flexneri O-antigen polymerase Wzy: identification of Wzz-dependent Wzy mutants.福氏志贺菌O抗原聚合酶Wzy的突变分析：Wzz依赖性Wzy突变体的鉴定
J Bacteriol. 2015 Jan 1;197(1):108-19. doi: 10.1128/JB.01885-14. Epub 2014 Oct 13.
9
An intramolecular chaperone inserted in bacteriophage P22 coat protein mediates its chaperonin-independent folding.一种插入噬菌体 P22 外壳蛋白的分子内伴侣介导了其伴侣蛋白独立折叠。
J Biol Chem. 2013 Nov 22;288(47):33772-33783. doi: 10.1074/jbc.M113.515312. Epub 2013 Oct 13.
10
RidA proteins prevent metabolic damage inflicted by PLP-dependent dehydratases in all domains of life.RidA 蛋白可防止所有生命领域中依赖 PLP 的脱水酶造成的代谢损伤。
mBio. 2013 Feb 5;4(1):e00033-13. doi: 10.1128/mBio.00033-13.
本文引用的文献
1
Inhibition of Escherichia coli viability by external guide sequences complementary to two essential genes.与两个必需基因互补的外部引导序列对大肠杆菌生存能力的抑制作用。
Proc Natl Acad Sci U S A. 2001 Jun 5;98(12):6605-10. doi: 10.1073/pnas.121180398. Epub 2001 May 29.
2
Inactivation of gene expression using ribonuclease P and external guide sequences.利用核糖核酸酶P和外部引导序列使基因表达失活
Methods Enzymol. 2000;313:442-56. doi: 10.1016/s0076-6879(00)13028-9.
3
Analysis of the functional role of conserved residues in the protein subunit of ribonuclease P from Escherichia coli.大肠杆菌核糖核酸酶P蛋白亚基中保守残基的功能作用分析。
J Mol Biol. 1997 Apr 11;267(4):818-29. doi: 10.1006/jmbi.1997.0906.
4
Overproduction of the toxic protein, bovine pancreatic DNaseI, in Escherichia coli using a tightly controlled T7-promoter-based vector.使用基于严格控制的T7启动子的载体在大肠杆菌中过量生产有毒蛋白牛胰脱氧核糖核酸酶I。
Gene. 1993 Dec 22;136(1-2):337-40. doi: 10.1016/0378-1119(93)90491-k.
5
The Salmonella typhimurium invasion genes invF and invG encode homologues of the AraC and PulD family of proteins.鼠伤寒沙门氏菌侵袭基因invF和invG编码AraC和PulD蛋白家族的同源物。
Mol Microbiol. 1994 Aug;13(4):555-68. doi: 10.1111/j.1365-2958.1994.tb00450.x.
6
Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter.由含有阿拉伯糖PBAD启动子的载体进行严格调控、调节和高水平表达。
J Bacteriol. 1995 Jul;177(14):4121-30. doi: 10.1128/jb.177.14.4121-4130.1995.
7
Artificial regulation of gene expression in Escherichia coli by RNase P.核糖核酸酶P对大肠杆菌基因表达的人工调控。
Proc Natl Acad Sci U S A. 1995 Nov 21;92(24):11115-9. doi: 10.1073/pnas.92.24.11115.
8
Nucleotide sequence of the gene for bacteriophage T7 RNA polymerase.噬菌体T7 RNA聚合酶基因的核苷酸序列。
J Mol Biol. 1984 Feb 25;173(2):265-9. doi: 10.1016/0022-2836(84)90194-3.
9
Positive selection for loss of tetracycline resistance.对四环素抗性丧失的正向选择。
J Bacteriol. 1980 Aug;143(2):926-33. doi: 10.1128/jb.143.2.926-933.1980.
10
Nucleotide sequence of the gene encoding the RNA subunit (M1 RNA) of ribonuclease P from Escherichia coli.编码来自大肠杆菌的核糖核酸酶P的RNA亚基（M1 RNA）的基因的核苷酸序列。
Cell. 1982 Sep;30(2):627-36. doi: 10.1016/0092-8674(82)90259-8.
Zotero 插件