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鼠伤寒沙门氏菌血清型鼠伤寒亚种启动子在利福平亚抑菌浓度水平下的转录调控

Transcription modulation of Salmonella enterica serovar Typhimurium promoters by sub-MIC levels of rifampin.

作者信息

Yim Grace, de la Cruz Fernando, Spiegelman George B, Davies Julian

机构信息

Department of Microbiology and Immunology, University of British Columbia, Life Sciences Institute, 2350 Health Sciences Mall, Vancouver, British Columbia V6T 1Z3, Canada.

出版信息

J Bacteriol. 2006 Nov;188(22):7988-91. doi: 10.1128/JB.00791-06. Epub 2006 Sep 15.

Abstract

Promoter-lux fusions that showed rifampin-modulated transcription were identified from a Salmonella enterica serovar Typhimurium 14028 reporter library. The transformation of a subset of fusions into mutants that lacked one of six global regulatory proteins or were rifampin resistant showed that transcription modulation was independent of the global regulators, promoter specific, and dependent on the interaction of rifampin with RNA polymerase.

摘要

从鼠伤寒沙门氏菌14028报告基因文库中鉴定出显示利福平调节转录的启动子-lux融合体。将一部分融合体转化为缺乏六种全局调节蛋白之一或对利福平耐药的突变体,结果表明转录调节独立于全局调节因子,具有启动子特异性,并且依赖于利福平与RNA聚合酶的相互作用。

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