一种绿色非硫细菌，脱卤球菌属产乙烯菌，具有在革兰氏阳性生物中发现的LexA结合序列。
A green nonsulfur bacterium, Dehalococcoides ethenogenes, with the LexA binding sequence found in gram-positive organisms.
作者信息
Fernández de Henestrosa Antonio R, Cuñé Jordi, Erill Ivan, Magnuson Jon K, Barbé Jordi
机构信息
Departament Genètica i Microbiologia, Universitat Autònoma de Barcelona, Spain.
出版信息
J Bacteriol. 2002 Nov;184(21):6073-80. doi: 10.1128/JB.184.21.6073-6080.2002.
Abstract
Dehalococcoides ethenogenes is a member of the physiologically diverse division of green nonsulfur bacteria. Using a TBLASTN search, the D. ethenogenes lexA gene has been identified, cloned, and expressed and its protein has been purified. Mobility shift assays revealed that the D. ethenogenes LexA protein specifically binds to both its own promoter and that of the uvrA gene, but not to the recA promoter. Our results demonstrate that the D. ethenogenes LexA binding site is GAACN(4)GTTC, which is identical to that found in gram-positive bacteria. In agreement with this fact, the Bacillus subtilis DinR protein binds specifically to the D. ethenogenes LexA operator. This constitutes the first non-gram-positive bacterium exhibiting a LexA binding site identical to that of B. subtilis.
摘要
嗜乙烯脱卤球菌是生理特性多样的绿色非硫细菌分类中的一员。通过TBLASTN搜索，已鉴定、克隆并表达了嗜乙烯脱卤球菌的lexA基因，其蛋白质也已纯化。迁移率变动分析表明，嗜乙烯脱卤球菌LexA蛋白能特异性结合其自身启动子以及uvrA基因的启动子，但不与recA启动子结合。我们的结果表明，嗜乙烯脱卤球菌LexA结合位点为GAACN(4)GTTC，这与革兰氏阳性菌中的相同。与此事实相符的是，枯草芽孢杆菌的DinR蛋白能特异性结合嗜乙烯脱卤球菌的LexA操纵基因。这是首个被发现具有与枯草芽孢杆菌相同LexA结合位点的非革兰氏阳性菌。
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