野生型和SOS缺陷型大肠杆菌紫外线照射后的基因表达谱比较
Comparative gene expression profiles following UV exposure in wild-type and SOS-deficient Escherichia coli.
作者信息
Courcelle J, Khodursky A, Peter B, Brown P O, Hanawalt P C
机构信息
Department of Biochemistry, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
出版信息
Genetics. 2001 May;158(1):41-64. doi: 10.1093/genetics/158.1.41.
The SOS response in UV-irradiated Escherichia coli includes the upregulation of several dozen genes that are negatively regulated by the LexA repressor. Using DNA microarrays containing amplified DNA fragments from 95.5% of all open reading frames identified on the E. coli chromosome, we have examined the changes in gene expression following UV exposure in both wild-type cells and lexA1 mutants, which are unable to induce genes under LexA control. We report here the time courses of expression of the genes surrounding the 26 documented lexA-regulated regions on the E. coli chromosome. We observed 17 additional sites that responded in a lexA-dependent manner and a large number of genes that were upregulated in a lexA-independent manner although upregulation in this manner was generally not more than twofold. In addition, several transcripts were either downregulated or degraded following UV irradiation. These newly identified UV-responsive genes are discussed with respect to their possible roles in cellular recovery following exposure to UV irradiation.
紫外线照射的大肠杆菌中的SOS反应包括上调几十个受LexA阻遏物负调控的基因。我们使用了包含从大肠杆菌染色体上鉴定出的所有开放阅读框的95.5%的扩增DNA片段的DNA微阵列，研究了野生型细胞和lexA1突变体在紫外线照射后基因表达的变化，lexA1突变体无法诱导受LexA控制的基因。我们在此报告大肠杆菌染色体上26个已记录的LexA调控区域周围基因的表达时间进程。我们观察到另外17个以LexA依赖方式作出反应的位点，以及大量以LexA非依赖方式上调的基因，尽管这种上调方式通常不超过两倍。此外，一些转录本在紫外线照射后要么下调要么降解。我们讨论了这些新鉴定的紫外线反应基因在暴露于紫外线照射后细胞恢复中的可能作用。
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