Erill Ivan, Campoy Susana, Mazon Gerard, Barbé Jordi
Biomedical Applications Group, Centro Nacional de Microelectrónica, 08193 Bellaterra, Spain.
Nucleic Acids Res. 2006 Jan 10;34(1):66-77. doi: 10.1093/nar/gkj412. Print 2006.
Recently, a multiple gene cassette with mutagenic translation synthesis activity was identified and shown to be under LexA regulation in several proteobacteria species. In this work, we have traced down instances of this multiple gene cassette across the bacteria domain. Phylogenetic analyses show that this cassette has undergone several reorganizations since its inception in the actinobacteria, and that it has dispersed across the bacterial domain through a combination of vertical inheritance, lateral gene transfer and duplication. In addition, our analyses show that LexA regulation of this multiple gene cassette is persistent in all the phyla in which it has been detected, and suggest that this regulation is prompted by the combined activity of two of its constituent genes: a polymerase V homolog and an alpha subunit of the DNA polymerase III.
最近，一种具有诱变翻译合成活性的多基因盒被鉴定出来，并在几种变形菌门物种中显示受LexA调控。在这项工作中，我们追踪了细菌域中这种多基因盒的实例。系统发育分析表明，自其在放线菌中出现以来，这个基因盒经历了几次重组，并且通过垂直遗传、横向基因转移和复制的组合在细菌域中扩散。此外，我们的分析表明，这种多基因盒的LexA调控在所有检测到它的门中都是持续存在的，并表明这种调控是由其两个组成基因的联合活性引发的：一个聚合酶V同源物和DNA聚合酶III的一个α亚基。
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