Siqueira José F, Rôças Isabela N
Department of Endodontics, Estácio de Sá University, Rio de Janeiro, Brazil.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2002 Oct;94(4):494-8. doi: 10.1067/moe.2002.125202.
Dialister pneumosintes is a nonmotile, nonfermentative, non-spore-forming, obligately anaerobic, gram-negative bacillus that has been associated with some infections in the human body. A species-specific nested polymerase chain reaction assay was used to investigate the occurrence of D pneumosintes in primary root canal infections.
Samples were collected from 32 teeth with carious lesions, necrotic pulps, and radiographic evidence of periradicular bone destruction. Twenty-two teeth were asymptomatic, and 10 cases were diagnosed as acute apical periodontitis. DNA extracted from the samples was initially amplified with universal 16S ribosomal DNA primers. A second round of amplification used the first polymerase chain reaction products to specifically detect D pneumosintes.
This bacterial species was detected in 17 of 22 asymptomatic cases (77.3%) and in 4 of 10 root canals associated with acute apical periodontitis (40%). No relationship was found between the presence of this bacterial species and the occurrence of symptoms. In general, D pneumosintes was detected in 21 of 32 root canal samples (65.6%).
This study is the first to report a high prevalence of D pneumosintes in root canal infections of humans. Because of this high prevalence and the apparent pathogenicity of the microorganism, we suggest the inclusion of D pneumosintes in the selected group of putative endodontic pathogens.
嗜肺 Dialister 菌是一种无动力、不发酵、不形成芽孢、专性厌氧的革兰氏阴性杆菌，与人体的一些感染有关。采用种特异性巢式聚合酶链反应分析法研究嗜肺 Dialister 菌在原发性根管感染中的发生情况。
从 32 颗有龋损、牙髓坏死且有根尖周骨破坏影像学证据的牙齿中采集样本。22 颗牙齿无症状，10 例被诊断为急性根尖周炎。从样本中提取的 DNA 首先用通用的 16S 核糖体 DNA 引物进行扩增。第二轮扩增使用第一轮聚合酶链反应产物来特异性检测嗜肺 Dialister 菌。
在 22 例无症状病例中的 17 例（77.3%）以及与急性根尖周炎相关的 10 个根管中的 4 个（40%）检测到了这种细菌。未发现该细菌的存在与症状的发生之间存在关联。总体而言，在 32 个根管样本中的 21 个（65.6%）检测到了嗜肺 Dialister 菌。
本研究首次报道了嗜肺 Dialister 菌在人类根管感染中的高流行率。鉴于这种高流行率以及该微生物明显的致病性，我们建议将嗜肺 Dialister 菌纳入选定的假定牙髓病病原体组。
