Zimmerman Robert L, Das Kiron M, Fogt Franz, Burke Melissa, Murakata Linda A
Department of Pathology W-547, Presbyterian Medical Center, Philadelphia, PA 19104, USA.
Oncol Rep. 2002 Nov-Dec;9(6):1369-72.
The distinction between cholangiocarcinoma, hepatocellular carcinoma and liver metastasis is important but at times difficult solely on microscopic appearances. The Das-1 immunostain exhibits specificity for colonic epithelium. However, its staining of cholangiocarcinomas and hepatocellular carcinomas has not been extensively studied. We evaluated the staining properties of the Das-1 immunostain in cholangiocarcinoma and hepatocellular carcinoma. Forty-four cholangiocarcinomas, 16 hepatocellular carcinomas, 17 colon adenocarcinomas metastatic to the liver and 3 benign biliary tumors were studied. The cases were stained with the Das-1 antibody following deparaffinization and rehydration. The slides were evaluated for membranous and/or cytoplasmic staining in a blinded fashion. The percentage of tumor cells exhibiting strong staining was estimated. Of the intra-hepatic cholangiocarcinomas, 4 of 36 (11%) and 2 of 16 (13%) hepatocellular carcinomas were positive for Das-1, though typically only in rare cells. In comparison, 7 of 8 (88%) extra-hepatic cholangiocarcinomas, 13 of 17 (77%) of metastatic colon carcinomas and 3 of 3 (100%) benign tumors of the biliary tract were strongly positive in a diffuse pattern. The Das-1 immunostain may play a useful role in evaluating liver malignancies.
胆管癌、肝细胞癌和肝转移瘤之间的鉴别很重要，但有时仅依靠显微镜下表现很难区分。Das-1免疫染色对结肠上皮具有特异性。然而，其在胆管癌和肝细胞癌中的染色情况尚未得到广泛研究。我们评估了Das-1免疫染色在胆管癌和肝细胞癌中的染色特性。研究了44例胆管癌、16例肝细胞癌、17例转移至肝脏的结肠腺癌以及3例良性胆管肿瘤。对这些病例进行脱蜡和水化处理后，用Das-1抗体进行染色。以盲法评估切片的膜性和/或细胞质染色情况。估计显示强染色的肿瘤细胞百分比。在肝内胆管癌中，36例中有4例（11%）、肝细胞癌16例中有2例（13%）Das-1呈阳性，但通常仅在少数细胞中。相比之下，8例肝外胆管癌中有7例（88%）、17例转移结肠癌中有13例（77%）以及3例胆管良性肿瘤中有3例（100%）呈弥漫性强阳性。Das-1免疫染色在评估肝脏恶性肿瘤方面可能发挥有用的作用。
