Chakrabarti Subhra, Bhattacharya Sumana, Bhattacharya Sanjoy K
Environmental Biotechnology Division, ABRD Company LLC, 1555 Wood Road, Cleveland, OH 44121, USA.
J Biochem Biophys Methods. 2002 Aug-Sep;52(3):179-87. doi: 10.1016/s0165-022x(02)00072-6.
A sensitive and nonradioactive assay method for activity determination of Rubisco is described. The method is based on thin-layer chromatographic separation of 3-phosphoglycerate (3-PGA) and D-ribulose-1,5-bisphosphate (RuBP). This assay method allows the quantitative determination of Rubisco activity. Rates of carbon dioxide fixation on RuBP determined by this method were comparable to those obtained independently by other methods. This assay method is reproducible and relatively free from interference.
本文描述了一种用于测定核酮糖-1,5-二磷酸羧化酶（Rubisco）活性的灵敏且非放射性的检测方法。该方法基于对3-磷酸甘油酸（3-PGA）和D-核酮糖-1,5-二磷酸（RuBP）的薄层色谱分离。这种检测方法能够对Rubisco活性进行定量测定。通过该方法测定的RuBP上二氧化碳固定速率与通过其他方法独立获得的速率相当。这种检测方法具有可重复性且相对不受干扰。
