Cabrera Marta, Canova Sergio, Rosenzvit Mara, Guarnera Eduardo
Departamento de Parasitologi;a. Instituto Nacional de Enfermedades Infecciosas "Dr Carlos G Malbrán," Av Vélez Sársfield 563 (1281), Buenos Aires, Argentina.
Diagn Microbiol Infect Dis. 2002 Sep;44(1):29-34. doi: 10.1016/s0732-8893(02)00414-5.
The eggs from Echinococcus granulosus contaminate the environment spreading out the disease among the herbivorous. The differential diagnosis of the embriophores recovered from the soil is very difficult by morphologic and immunologic methods. In this paper we evaluate the EgO/DNA-IM1 for identification of E. granulosus oncosphere DNA and differentiation of eggs from other Taeniid. The positive result of the PCR technique shows an amplification fragment of the expected size (285 bp) corresponding to the partial sequence of the mitochondrial gene of the cytochrome oxidase CO1 from E. granulosus (391 bp). The fragment is not present in the DNA from Echinococcus multilocularis, Taenia hydatigena, Taenia saginata, Diphyll-obothrium latum, and Hymenolepis nana. It could be useful to rule out Taenia taeniformis, Taenia solium, Taenia pisiformis, and Taenia crassiceps, which sequences do not belong to the primer. We concluded that the PCR amplification employing the EgO/DNA-IM1 primer set showed high sensitivity and specificity for the identification of Echinococcus granulosus eggs.
细粒棘球绦虫的虫卵污染环境，在食草动物中传播疾病。通过形态学和免疫学方法对从土壤中回收的胚体进行鉴别诊断非常困难。在本文中，我们评估了EgO/DNA-IM1用于鉴定细粒棘球绦虫原头蚴DNA以及区分其虫卵与其他带绦虫虫卵的能力。PCR技术的阳性结果显示出预期大小（285 bp）的扩增片段，对应于细粒棘球绦虫细胞色素氧化酶CO1线粒体基因的部分序列（391 bp）。该片段不存在于多房棘球绦虫、有钩绦虫、牛带绦虫、阔节裂头绦虫和微小膜壳绦虫的DNA中。它可能有助于排除泡状带绦虫、猪带绦虫、豆状带绦虫和肥胖带绦虫，因为它们的序列与引物不匹配。我们得出结论，使用EgO/DNA-IM1引物组进行PCR扩增对细粒棘球绦虫虫卵的鉴定具有高灵敏度和特异性。
