Stefanić Sasa, Shaikenov Block S, Deplazes Peter, Dinkel Anke, Torgerson Paul R, Mathis Alexander
Institute of Parasitology, University of Zürich, Winterthurerstrasse 266a, 8057 Zürich, Switzerland.
Parasitol Res. 2004 Mar;92(4):347-51. doi: 10.1007/s00436-003-1043-y. Epub 2004 Jan 16.
Polymerase chain reaction (PCR) for the identification of eggs of the tapeworm Echinococcus granulosus ("sheep strain") was evaluated with primers derived from mitochondrial sequences. Specificity of these primers was confirmed by investigating DNA of other strains of E. granulosus and of 14 helminth species which inhabit the intestines of dogs. This PCR assay was used to investigate 131 purged dogs from Kazakhstan. Eighteen dogs harboured Echinococcus worms, ten of them in mixed infections with Taenia spp. Coproantigen detection was positive in 15 and taeniid eggs could be recovered from 13 of these specimens. Eight of the egg-containing samples were positive in the PCR for E. granulosus and four in a Echinococcus multilocularis -specific PCR revealing one mixed infection. Egg-containing faeces from two dogs harbouring both Taenia spp. and Echinococcus spp. were negative in both PCRs. The combination of egg isolation and PCR will also be of value in epidemiological studies when investigating environmental samples.
利用源自线粒体序列的引物，对用于鉴定细粒棘球绦虫（“羊株”）虫卵的聚合酶链反应（PCR）进行了评估。通过研究细粒棘球绦虫其他菌株以及寄生于犬肠道的14种蠕虫的DNA，证实了这些引物的特异性。该PCR检测方法用于检测来自哈萨克斯坦的131只驱虫犬。18只犬感染了棘球绦虫，其中10只与带绦虫属混合感染。15份样本的粪抗原检测呈阳性，其中13份样本可检出带绦虫属虫卵。8份含虫卵样本的细粒棘球绦虫PCR检测呈阳性，4份在多房棘球绦虫特异性PCR检测中呈阳性，显示有1例混合感染。两只同时感染带绦虫属和棘球绦虫属的犬的含虫卵粪便在两种PCR检测中均为阴性。在调查环境样本时，虫卵分离和PCR相结合在流行病学研究中也将具有重要价值。
