Torres Iraci Luena S, Buffon Andreia, Dantas Giovana, Fürstenau Cristina Ribas, Böhmer Ana Elisa, Battastini Ana Mario O, Sarkis João J F, Dalmaz Carla, Ferreira Maria Beatriz C
Departamento de Bioqui;mica, Instituto de Ciências Básicas da Saúde, UFRGS, Ramiro Barcelos, 2600 Anexo, Lab. 32, 90035-003, RS, Porto Alegre, Brazil
Pharmacol Biochem Behav. 2002 Dec;74(1):181-6. doi: 10.1016/s0091-3057(02)00994-2.
We have previously observed that adenosine 5'-diphosphate (ADP) hydrolysis was decreased 25% in spinal cord synaptosomes of chronically stressed male rats, while no changes were observed in ATPase activity. In the present study, we investigated the effect of chronic stress on the hydrolysis of adenine nucleotides in two cerebral structures (frontal cortex and hypothalamus) and in the blood serum of male rats. Adult male Wistar rats were submitted to 1-h restraint stress/day for 45 days (chronic) and were sacrificed 24 h after the last session of stress. Adenosine 5'-triphosphate (ATP) or ADP hydrolysis was assayed in the synaptosomal fraction obtained from the frontal cortex and hypothalamus of control and chronically stressed animals. No effects on ADP or ATP hydrolysis were observed in any of the cerebral structures analyzed after chronic stress. On the other hand, reduced ADP hydrolysis was observed in the blood serum of chronic stressed rats. It is possible that the effects observed in the blood serum may represent an adaptation to chronic stress and may reflect different functions of nucleotides and/or enzymes in these tissues. It is possible that altered levels of ADPase activity in the serum may be a biochemical marker for chronic stress situations.
我们之前观察到，在长期应激的雄性大鼠脊髓突触体中，5'-二磷酸腺苷（ADP）水解降低了25%，而三磷酸腺苷酶（ATPase）活性未观察到变化。在本研究中，我们调查了长期应激对雄性大鼠两个脑区结构（额叶皮质和下丘脑）以及血清中腺嘌呤核苷酸水解的影响。成年雄性Wistar大鼠每天接受1小时束缚应激，持续45天（长期应激），并在最后一次应激结束后24小时处死。对对照组和长期应激动物的额叶皮质和下丘脑获得的突触体部分进行5'-三磷酸腺苷（ATP）或ADP水解测定。长期应激后，在所分析的任何脑区结构中均未观察到对ADP或ATP水解的影响。另一方面，在长期应激大鼠的血清中观察到ADP水解减少。血清中观察到的影响可能代表对长期应激的一种适应，并且可能反映这些组织中核苷酸和/或酶的不同功能。血清中ADP酶活性的改变水平可能是长期应激状态的生化标志物。
