Harp Joyce B, Henry Steven A, DiGirolamo Mario
Department of Medicine, Emory University School of Medicine, Atlanta, Georgia, USA.
Obes Res. 2002 Oct;10(10):985-90. doi: 10.1038/oby.2002.134.
To study the effect of dietary weight loss, postural change, and an oral glucose load on serum angiotensin-converting enzyme (ACE) activity in obese adults.
Sixteen obese adult men and women with a mean body mass index of 35.7 +/- 4.3 kg/m(2) were studied after 1 week on a maintenance energy lead-in diet and after 5 weeks on an identical but 40% reduced-energy diet provided by the General Clinical Research Center (GCRC). ACE activity was measured spectrophotometrically. Plasma renin activity and serum aldosterone were measured by radioimmunoassay.
All subjects lost weight, with a mean decrease in body weight of 7.0 +/- 2.1 kg or 6 +/- 3% of initial body weight (p < 0.00001). Systolic and diastolic blood pressure, supine plasma renin activity, and serum aldosterone levels decreased with weight loss (p < 0.05). Supine ACE activity decreased 23 +/- 12% with weight loss (p < 0.00001). Standing ACE activity, which was significantly higher than supine ACE activity before and after weight loss (p < 0.05), also decreased 18 +/- 17% with weight loss (p = 0.0007). A 75-g oral glucose load had no effect on serum ACE activity over a 3-hour period.
In obese adults, serum ACE activity declines with modest weight loss, increases with postural change, and is unaffected by an oral glucose load.
研究饮食减肥、体位改变和口服葡萄糖负荷对肥胖成年人血清血管紧张素转换酶（ACE）活性的影响。
16名肥胖成年男性和女性，平均体重指数为35.7±4.3kg/m²，先在普通临床研究中心（GCRC）进行1周维持能量导入饮食，之后进行5周相同但能量减少40%的饮食。采用分光光度法测量ACE活性，通过放射免疫分析法测量血浆肾素活性和血清醛固酮。
所有受试者体重均减轻，平均体重下降7.0±2.1kg，占初始体重的6±3%（p<0.00001）。收缩压和舒张压、仰卧位血浆肾素活性及血清醛固酮水平随体重减轻而下降（p<0.05）。仰卧位ACE活性随体重减轻下降23±12%（p<0.00001）。站立位ACE活性在体重减轻前后均显著高于仰卧位（p<0.05），也随体重减轻下降18±17%（p=0.0007）。75g口服葡萄糖负荷在3小时内对血清ACE活性无影响。
在肥胖成年人中，血清ACE活性随适度体重减轻而下降，随体位改变而升高，且不受口服葡萄糖负荷影响。
