异染色质蛋白2（HP2），果蝇异染色质中HP1的一个伴侣蛋白。
Heterochromatin protein 2 (HP2), a partner of HP1 in Drosophila heterochromatin.
作者信息
Shaffer Christopher D, Stephens Gena E, Thompson Brandi A, Funches Levi, Bernat John A, Craig Carolyn A, Elgin Sarah C R
机构信息
Department of Biology, Washington University, CB-1229, St. Louis, MO 63130, USA.
出版信息
Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14332-7. doi: 10.1073/pnas.212458899. Epub 2002 Oct 10.
Abstract
Heterochromatin protein 1 (HP1), first discovered in Drosophila melanogaster, is a highly conserved chromosomal protein implicated in both heterochromatin formation and gene silencing. We report here characterization of an HP1-interacting protein, heterochromatin protein 2 (HP2), which codistributes with HP1 in the pericentric heterochromatin. HP2 is a large protein with two major isoforms of approximately 356 and 176 kDa. The smaller isoform is produced from an alternative splicing pattern in which two exons are skipped. Both isoforms contain the domain that interacts with HP1; the larger isoform contains two AT-hook motifs. Mutations recovered in HP2 act as dominant suppressors of position effect variegation, confirming a role in heterochromatin spreading and gene silencing.
摘要
异染色质蛋白1（HP1）最初在黑腹果蝇中发现，是一种高度保守的染色体蛋白，与异染色质形成和基因沉默均有关联。我们在此报告一种与HP1相互作用的蛋白——异染色质蛋白2（HP2）的特征，它与HP1在着丝粒周围异染色质中共分布。HP2是一种大型蛋白，有两种主要的异构体，大小约为356 kDa和176 kDa。较小的异构体由一种可变剪接模式产生，其中两个外显子被跳过。两种异构体都含有与HP1相互作用的结构域；较大的异构体含有两个AT钩基序。在HP2中发现的突变可作为位置效应斑驳的显性抑制因子，证实其在异染色质扩展和基因沉默中的作用。
相似文献
1
Heterochromatin protein 2 (HP2), a partner of HP1 in Drosophila heterochromatin.异染色质蛋白2（HP2），果蝇异染色质中HP1的一个伴侣蛋白。
Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14332-7. doi: 10.1073/pnas.212458899. Epub 2002 Oct 10.
2
The large isoform of Drosophila melanogaster heterochromatin protein 2 plays a critical role in gene silencing and chromosome structure.果蝇异染色质蛋白2的大型异构体在基因沉默和染色体结构中起关键作用。
Genetics. 2006 Nov;174(3):1189-204. doi: 10.1534/genetics.106.057604. Epub 2006 Sep 15.
3
Heterochromatin protein 2 interacts with Nap-1 and NURF: a link between heterochromatin-induced gene silencing and the chromatin remodeling machinery in Drosophila.异染色质蛋白2与Nap-1和NURF相互作用：果蝇中异染色质诱导的基因沉默与染色质重塑机制之间的联系。
Biochemistry. 2006 Dec 19;45(50):14990-9. doi: 10.1021/bi060983y.
4
Interaction of heterochromatin protein 2 with HP1 defines a novel HP1-binding domain.异染色质蛋白2与HP1的相互作用定义了一个新的HP1结合结构域。
Biochemistry. 2005 Oct 11;44(40):13394-403. doi: 10.1021/bi051006+.
5
The genomic silencing of position-effect variegation in Drosophila melanogaster: interaction between the heterochromatin-associated proteins Su(var)3-7 and HP1.黑腹果蝇中位置效应斑驳的基因组沉默：异染色质相关蛋白Su(var)3-7与HP1之间的相互作用
J Cell Sci. 2000 Dec;113 Pt 23:4253-61. doi: 10.1242/jcs.113.23.4253.
6
Hip, an HP1-interacting protein, is a haplo- and triplo-suppressor of position effect variegation.Hip是一种与HP1相互作用的蛋白质，是位置效应斑驳的单倍体和三倍体抑制因子。
Proc Natl Acad Sci U S A. 2008 Jan 8;105(1):204-9. doi: 10.1073/pnas.0705595105. Epub 2007 Dec 27.
7
Umbrea, a chromo shadow domain protein in Drosophila melanogaster heterochromatin, interacts with Hip, HP1 and HOAP.Umbrea是黑腹果蝇异染色质中的一种染色体阴影结构域蛋白，它与Hip、HP1和HOAP相互作用。
Chromosome Res. 2009;17(1):19-36. doi: 10.1007/s10577-008-9002-1. Epub 2009 Feb 4.
8
Heterochromatic silencing and HP1 localization in Drosophila are dependent on the RNAi machinery.果蝇中的异染色质沉默和HP1定位依赖于RNA干扰机制。
Science. 2004 Jan 30;303(5658):669-72. doi: 10.1126/science.1092653.
9
HP2-like protein: a new piece of the facultative heterochromatin puzzle.类HP2蛋白：兼性异染色质谜题的新拼图块。
Chromosoma. 2007 Jun;116(3):249-58. doi: 10.1007/s00412-007-0095-7. Epub 2007 Feb 13.
10
Positive regulation of euchromatic gene expression by HP1.异染色质蛋白1对常染色质基因表达的正向调控
Fly (Austin). 2010 Oct-Dec;4(4):299-301. doi: 10.4161/fly.4.4.13261. Epub 2010 Oct 3.
引用本文的文献
1
A nuclear architecture screen in identifies Stonewall as a link between chromatin position at the nuclear periphery and germline stem cell fate.一项核架构筛选发现，Stonewall 是核周染色质位置与生殖干细胞命运之间的联系。
Genes Dev. 2024 Jun 25;38(9-10):415-435. doi: 10.1101/gad.351424.123.
2
Inheritance of H3K9 methylation regulates genome architecture in Drosophila early embryos.H3K9 甲基化的遗传调控果蝇早期胚胎中的基因组结构。
EMBO J. 2024 Jul;43(13):2685-2714. doi: 10.1038/s44318-024-00127-z. Epub 2024 Jun 3.
3
A nuclear architecture screen in identifies Stonewall as a link between chromatin position at the nuclear periphery and germline stem cell fate.一项细胞核结构筛选确定了“石墙”蛋白是核周染色质位置与生殖系干细胞命运之间的一个联系纽带。
bioRxiv. 2023 Nov 17:2023.11.17.567611. doi: 10.1101/2023.11.17.567611.
4
Molecular Mechanisms for the Regulation of Nuclear Membrane Integrity.核膜完整性调控的分子机制。
Int J Mol Sci. 2023 Oct 23;24(20):15497. doi: 10.3390/ijms242015497.
5
Genome-Wide Identification of Long Noncoding RNA and Their Potential Interactors in Mutants.全基因组鉴定突变体中的长非编码 RNA 及其潜在相互作用物。
Int J Mol Sci. 2022 Jun 2;23(11):6247. doi: 10.3390/ijms23116247.
6
Insights into HP1a-Chromatin Interactions.HP1a-染色质相互作用的研究进展。
Cells. 2020 Aug 9;9(8):1866. doi: 10.3390/cells9081866.
7
Genetic Variation and Potential for Resistance Development to the tTA Overexpression Lethal System in Insects.昆虫中tTA过表达致死系统的遗传变异及抗性发展潜力
G3 (Bethesda). 2020 Apr 9;10(4):1271-1281. doi: 10.1534/g3.120.400990.
8
The Dot Chromosome: Where Genes Flourish Amidst Repeats.着丝粒染色体：基因在重复序列中茁壮成长的地方。
Genetics. 2018 Nov;210(3):757-772. doi: 10.1534/genetics.118.301146.
9
In praise of mealybugs.赞美粉蚧。
J Genet. 2018 Jun;97(2):379-389.
10
Visualization of the Genomic Loci That Are Bound by Specific Multiprotein Complexes by Bimolecular Fluorescence Complementation Analysis on Drosophila Polytene Chromosomes.通过果蝇多线染色体上的双分子荧光互补分析对特定多蛋白复合物结合的基因组位点进行可视化。
Methods Enzymol. 2017;589:429-455. doi: 10.1016/bs.mie.2017.02.003. Epub 2017 Mar 11.
本文引用的文献
1
Epigenetic codes for heterochromatin formation and silencing: rounding up the usual suspects.异染色质形成与沉默的表观遗传密码：搜罗常见因素。
Cell. 2002 Feb 22;108(4):489-500. doi: 10.1016/s0092-8674(02)00644-x.
2
Central role of Drosophila SU(VAR)3-9 in histone H3-K9 methylation and heterochromatic gene silencing.果蝇SU(VAR)3-9在组蛋白H3-K9甲基化和异染色质基因沉默中的核心作用。
EMBO J. 2002 Mar 1;21(5):1121-31. doi: 10.1093/emboj/21.5.1121.
3
The AT-hook protein D1 is essential for Drosophila melanogaster development and is implicated in position-effect variegation.AT钩蛋白D1对黑腹果蝇的发育至关重要，并与位置效应斑驳有关。
Mol Cell Biol. 2002 Feb;22(4):1218-32. doi: 10.1128/MCB.22.4.1218-1232.2002.
4
Recruitment of cohesin to heterochromatic regions by Swi6/HP1 in fission yeast.裂殖酵母中Swi6/HP1将黏连蛋白招募至异染色质区域。
Nat Cell Biol. 2002 Jan;4(1):89-93. doi: 10.1038/ncb739.
5
Requirement of heterochromatin for cohesion at centromeres.着丝粒处黏连对异染色质的需求。
Science. 2001 Dec 21;294(5551):2539-42. doi: 10.1126/science.1064027. Epub 2001 Oct 11.
6
Transcriptional repression of euchromatic genes by Drosophila heterochromatin protein 1 and histone modifiers.果蝇异染色质蛋白1和组蛋白修饰剂对常染色质基因的转录抑制作用。
Proc Natl Acad Sci U S A. 2001 Sep 25;98(20):11423-7. doi: 10.1073/pnas.211303598. Epub 2001 Sep 18.
7
Drosophila heterochromatin protein 1 (HP1)/origin recognition complex (ORC) protein is associated with HP1 and ORC and functions in heterochromatin-induced silencing.果蝇异染色质蛋白1（HP1）/复制起始点识别复合物（ORC）蛋白与HP1和ORC相关联，并在异染色质诱导的基因沉默中发挥作用。
Mol Biol Cell. 2001 Jun;12(6):1671-85. doi: 10.1091/mbc.12.6.1671.
8
Long-range nucleosome ordering is associated with gene silencing in Drosophila melanogaster pericentric heterochromatin.远距离核小体排序与黑腹果蝇着丝粒周围异染色质中的基因沉默相关。
Mol Cell Biol. 2001 Apr;21(8):2867-79. doi: 10.1128/MCB.21.8.2867-2879.2001.
9
Selective recognition of methylated lysine 9 on histone H3 by the HP1 chromo domain.HP1染色质结构域对组蛋白H3上甲基化赖氨酸9的选择性识别。
Nature. 2001 Mar 1;410(6824):120-4. doi: 10.1038/35065138.
10
Methylation of histone H3 lysine 9 creates a binding site for HP1 proteins.组蛋白H3赖氨酸9的甲基化产生了异染色质蛋白1（HP1）家族蛋白的结合位点。
Nature. 2001 Mar 1;410(6824):116-20. doi: 10.1038/35065132.
Zotero 插件