Li Y, Collins M S, Whitelam G C, Alexander D J
Department of Biology, University of Leicester, Leicester, UK.
Arch Virol. 2002 Oct;147(10):2025-37. doi: 10.1007/s00705-002-0862-y.
Filamentous bacteriophage display technology has been used to generate specific antibody fragments for differentiating virulent and avirulent Newcastle disease virus. A single-chain Fv fragment to the motif (112)RRQ(114), present at the F2 C-terminal end of many virulent Newcastle disease virus isolates, was isolated from a phage display library derived from a rabbit immunized with a peptide conjugate. An ELISA evaluation was carried out to test its ability to differentiate between 11 avirulent and 34 virulent NDV isolates. The antibody fragment reacted with 25/28 virulent viruses with the putative motif (112)RRQ(114). The three exceptions were viruses with an arginine instead of glycine, at position 110 of the fusion protein, just preceding the cleavage site. Five of six virulent isolates, whose predicted motif was different from that usually found in virulent strains, also tested negative. However, the antibody did react with one isolate with the motif (112)KRQ(114). There was no apparent reactivity with any of the avirulent isolates tested. We conclude that this antibody may, in the future, be a useful aid for the pathotyping of NDV isolates.
丝状噬菌体展示技术已被用于生成用于区分强毒和无毒新城疫病毒的特异性抗体片段。从用肽缀合物免疫的兔子来源的噬菌体展示文库中分离出一种针对许多强毒新城疫病毒分离株F2 C末端基序（112）RRQ（114）的单链Fv片段。进行了酶联免疫吸附测定（ELISA）以测试其区分11种无毒和34种强毒新城疫病毒分离株的能力。该抗体片段与25/28种具有推定基序（112）RRQ（114）的强毒病毒发生反应。三个例外是融合蛋白110位（紧接裂解位点之前）为精氨酸而非甘氨酸的病毒。六个预测基序与通常在强毒株中发现的基序不同的强毒分离株中有五个也呈阴性反应。然而，该抗体确实与一种具有基序（112）KRQ（114）的分离株发生反应。对所测试的任何无毒分离株均无明显反应性。我们得出结论，这种抗体将来可能有助于新城疫病毒分离株的致病型鉴定。
