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单链抗体片段的生产与应用。

The production and application of single-chain antibody fragments.

作者信息

Blazek D, Celer V

机构信息

Institute of Microbiology and Immunology, Faculty of Veterinary Medicine, Veterinary and Pharmaceutical University, 612 42 Brno, Czechia.

出版信息

Folia Microbiol (Praha). 2003;48(5):687-98. doi: 10.1007/BF02993480.

DOI:10.1007/BF02993480
PMID:14976730
Abstract

This review discusses methods for the single-chain antibody fragment ($cFv) generation and scFv expression systems, and describes potential applications of scFv in the therapy of viral diseases and cancer, with emphasis on intracellularly expressed scFvs (intrabodies), application of scFvs in detection and diagnostics, and their use in proteomics.

摘要

本综述讨论了单链抗体片段（scFv）的产生方法和scFv表达系统，并描述了scFv在病毒性疾病和癌症治疗中的潜在应用，重点介绍了细胞内表达的scFv（胞内抗体）、scFv在检测和诊断中的应用及其在蛋白质组学中的用途。

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本文引用的文献

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Application of Phage Display in Selecting Tomato spotted wilt virus-Specific Single-Chain Antibodies (scFvs) for Sensitive Diagnosis in ELISA.噬菌体展示技术在筛选番茄斑萎病毒特异性单链抗体（scFvs）用于 ELISA 灵敏诊断中的应用。
Phytopathology. 2000 Feb;90(2):183-90. doi: 10.1094/PHYTO.2000.90.2.183.
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Recombinant single-chain Fv antibodies that recognize the p25 protein of the Maedi-Visna virus.可识别梅迪-维斯纳病毒p25蛋白的重组单链Fv抗体。
Folia Microbiol (Praha). 2003;48(3):435-40. doi: 10.1007/BF02931380.
3
Neutralising human recombinant antibodies to human cytomegalovirus glycoproteins gB and gH.
全细胞淘选法成功应用于分离分化型胃癌细胞特异性噬菌体抗体片段
Adv Pharm Bull. 2019 Oct;9(4):624-631. doi: 10.15171/apb.2019.072. Epub 2019 Oct 24.
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Strategies for Selecting Membrane Protein-Specific Antibodies using Phage Display with Cell-Based Panning.利用基于细胞淘选的噬菌体展示技术筛选膜蛋白特异性抗体的策略
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Construction, expression and functional analysis of anti-B7-H4- scFv-CH3 recombinant antibody.抗B7-H4-scFv-CH3重组抗体的构建、表达及功能分析
J Biosci. 2018 Sep;43(4):661-671.
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Generation of recombinant antibodies to rat GABAA receptor subunits by affinity selection on synthetic peptides.通过对合成肽进行亲和选择产生针对大鼠γ-氨基丁酸A型（GABAA）受体亚基的重组抗体。
PLoS One. 2014 Feb 19;9(2):e87964. doi: 10.1371/journal.pone.0087964. eCollection 2014.
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Phage display on the base of filamentous bacteriophages: application for recombinant antibodies selection.噬菌体展示技术在丝状噬菌体基础上的应用：用于重组抗体的选择。
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