Tisch M, Bergenthal S, Maier H
Abteilung Hals-Nasen-Ohrenheilkunde, Kopf- und Halschirurgie, Bundeswehrkrankenhaus Ulm, Germany.
HNO. 2002 Oct;50(10):920-7. doi: 10.1007/s00106-001-0621-5.
Experimental and epidemiological studies have provided evidence that pentachlorophenol (PCP) and gamma-hexachlorocyclohexane (lindane) may pose a potential carcinogenic risk for human epithelial cells of the upper aerodigestive tract. In the past, these two substances have been used for military and nonmilitary purposes, e.g., for impregnation of textiles and uniforms. In this study, we investigated the genotoxic effect of PCP and lindane on human mucosal tissue from the tonsils.
In epithelia obtained from the tonsillar mucosa removed during surgery, cell viability was evaluated by trypan blue staining. The specimens were incubated for 60 min with PCP (0.3, 0.75, and 1.2 mM) and lindane (0.5, 0.75, and 1.0 mM). The induction of DNA damage (single- and double-strand breaks) caused by PCP and lindane was measured using single-cell microgel electrophoresis. Evaluation was performed with an image analyzer enhanced by fluorescence microscopy.
After exposure to PCP and lindane, strong genotoxic effects are apparent. The DNA migration rose from 26 micrometer in the control solution up to nearly 90 micrometer after incubation with PCP and lindane ( p<0.0001).
This study could demonstrate for the first time genotoxic effects of PCP and lindane on human tonsillar epithelium. It has to be considered that chronic exposure to both agents might increase the risk for cancer of the upper aerodigestive tract.
实验和流行病学研究已提供证据表明，五氯苯酚（PCP）和γ-六氯环己烷（林丹）可能对上呼吸道和消化道的人类上皮细胞构成潜在致癌风险。过去，这两种物质曾用于军事和非军事用途，例如用于纺织品和制服的浸渍。在本研究中，我们调查了PCP和林丹对扁桃体人类黏膜组织的遗传毒性作用。
在手术中切除的扁桃体黏膜上皮中，通过台盼蓝染色评估细胞活力。将标本与PCP（0.3、0.75和1.2 mM）和林丹（0.5、0.75和1.0 mM）孵育60分钟。使用单细胞微凝胶电泳测量PCP和林丹引起的DNA损伤（单链和双链断裂）诱导情况。通过荧光显微镜增强的图像分析仪进行评估。
暴露于PCP和林丹后，明显出现强烈的遗传毒性作用。DNA迁移从对照溶液中的26微米增加到与PCP和林丹孵育后的近90微米（p<0.0001）。
本研究首次证明了PCP和林丹对人类扁桃体上皮的遗传毒性作用。必须考虑到长期接触这两种物质可能会增加上呼吸道和消化道癌症的风险。
