Qiu De-Kai, Ma Xiong, Peng Yan-Shen, Chen Xiao-Yu
Shanghai Institute of Digestive Diseases, Renji Hospital, Shanghai Second Medical University, Shanghai, 200001, China.
World J Gastroenterol. 2002 Oct;8(5):815-7. doi: 10.3748/wjg.v8.i5.815.
To clarify the significance of cyclooxygenase-2 (COX-2) expression in human primary hepatocellular carcinoma (HCC) and adjacent nontumorous tissues.
The COX-2 protein and mRNA were investigated in 27 HCC tissues with adjacent nontumorous tissues, and 5 histologically normal liver tissues,using immunohistochemistry and in situ hybridization.
The well-differentiated HCC expressed COX-2 protein (5.68+/-1.19) more strongly than moderated HCC (3.43+/-1.98) and poor differentiated HCC (3.33+/-1.50) (P<0.05 respectively), adjacent nontumorous tissues (4.93+/-1.05) and normal liver tissues (3.20+/-1.92) (P<0.01 respectively); More intensive staining of COX-2 in adjacent nontumorous tissues was observed than that in normal liver tissues (P<0.05). There was no significant difference among adjacent nontumorous tissues, moderately differentiated HCC and poorly differentiated HCC (P>0.05). The expression of COX-2 mRNA was observed in the cytoplasm of the cells of HCC and of the hepatocytes in adjacent nontumorous tissues in which COX-2 protein was positive.
The overexpression of COX-2 in well-differentiated HCC suggests that COX-2 may play a role in the early stages of hepatocarcinogensis.
阐明环氧化酶-2（COX-2）在人原发性肝细胞癌（HCC）及癌旁非肿瘤组织中的表达意义。
采用免疫组织化学和原位杂交技术，对27例HCC组织及其癌旁非肿瘤组织，以及5例组织学正常的肝组织进行COX-2蛋白和mRNA检测。
高分化HCC组织中COX-2蛋白表达水平（5.68±1.19）明显高于中分化HCC组织（3.43±1.98）和低分化HCC组织（3.33±1.50）（P均<0.05），也高于癌旁非肿瘤组织（4.93±1.05）和正常肝组织（3.20±1.92）（P均<0.01）；癌旁非肿瘤组织中COX-2染色强度高于正常肝组织（P<0.05）。癌旁非肿瘤组织、中分化HCC组织和低分化HCC组织之间差异无统计学意义（P>0.05）。在COX-2蛋白阳性的HCC细胞及癌旁非肿瘤组织的肝细胞胞质中均观察到COX-2 mRNA的表达。
高分化HCC中COX-2的过表达提示COX-2可能在肝癌发生的早期阶段起作用。
