Affonso E G, Polez V L P, Corrêa C F, Mazon A F, Araújo M R R, Moraes G, Rantin F T
Department of Aquaculture, National Institute of Amazonian Research, Av. André Araújo, 2936, P.O. Box 478, 69083-000 Manaus, AM, Brazil.
Comp Biochem Physiol C Toxicol Pharmacol. 2002 Nov;133(3):375-82. doi: 10.1016/s1532-0456(02)00127-8.
Juvenile tambaqui, Colossoma macropomum, were exposed to sulfide and hypoxia for 12, 24, 48 and 96 h. Hemoglobin concentrations, red blood cell counts, and mean cell hemoglobin, were higher at 12 h in fish exposed to hypoxia. However, control fish and those exposed to sulfide and hypoxia had lower red blood cell count, hemoglobin concentration and hematocrit at 96 h. Methemoglobin was higher than in the controls, probably due to the hypoxemia induced by these stressors. Sulfhemoglobin was not detected in significant amounts in the blood of fish exposed to sulfide (in vivo), yet hemoglobin converted into sulfhemoglobin at 1-15 mM sulfide in vitro. Anaerobic metabolism seemed to be an important mechanism for adapting to sulfide exposure and blood pH returned to control values after 24 h of sulfide, preventing acidosis. The high sulfide tolerance in tambaqui is associated with its high tolerance to hypoxia.
将未成年的巨脂鲤（Colossoma macropomum）暴露于硫化物和低氧环境中12、24、48和96小时。暴露于低氧环境12小时的鱼，其血红蛋白浓度、红细胞计数和平均细胞血红蛋白含量较高。然而，对照鱼以及暴露于硫化物和低氧环境的鱼在96小时时红细胞计数、血红蛋白浓度和血细胞比容较低。高铁血红蛋白高于对照组，这可能是由于这些应激源引起的低氧血症所致。在暴露于硫化物的鱼的血液中（体内）未检测到大量的硫血红蛋白，但在体外，当硫化物浓度为1 - 15 mM时，血红蛋白会转化为硫血红蛋白。无氧代谢似乎是适应硫化物暴露的重要机制，在暴露于硫化物24小时后，血液pH值恢复到对照值，从而防止酸中毒。巨脂鲤对高硫化物的耐受性与其对低氧的高耐受性有关。
