Shahjahan Md, Khatun Most Sabia, Mun Mim Mostarin, Islam S M Majharul, Uddin Md Helal, Badruzzaman Muhammad, Khan Saleha
Laboratory of Fish Ecophysiology, Department of Fisheries Management, Bangladesh Agricultural University, Mymensingh, Bangladesh.
Department of Biochemistry and Molecular Biology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh.
Front Physiol. 2020 Jun 5;11:543. doi: 10.3389/fphys.2020.00543. eCollection 2020.
As a consequence of global warming, increase of water temperature is likely to alter physiological functions of fish. Hence, we examined the effects of high temperature on blood glucose, hematological parameters [hemoglobin (Hb), red blood cell (RBC), and white blood cell (WBC)], and nuclear and cellular structure of blood cells of common carp () after exposure to three temperature regimes (27, 31, and 35°C) for 14 days. Fish were sacrificed on 3, 7, and 14 days of exposure. The blood glucose level increased significantly in the fish exposed to 35°C compared to 27 and 31°C. The Hb and RBC contents decreased but WBC increased significantly in the blood of fish exposed to 35°C compared to 27 and 31°C at 7 and 14 days of exposure. Consequently, the frequencies of erythroblasts (Ebs), erythrocytic nuclear abnormalities (ENA), and erythrocytic cellular abnormalities (ECA) were found to be increased in the blood of fish exposed to 35°C compared to 27 and 31°C. There was a significant increase in neutrophils and decrease in lymphocytes in the highest temperature (35°C). With increasing temperature, dissolved oxygen (DO) decreased but free CO increased significantly during the study period. The present study demonstrated that common carp are better adapted to 27 and 31°C environmental temperatures, while the higher temperature 35°C is likely stressful to this fish species.
由于全球变暖，水温升高可能会改变鱼类的生理功能。因此，我们研究了在暴露于三种温度条件（27、31和35°C）14天后，高温对鲤鱼血糖、血液学参数[血红蛋白（Hb）、红细胞（RBC）和白细胞（WBC）]以及血细胞的细胞核和细胞结构的影响。在暴露后的第3、7和14天对鱼进行宰杀。与27°C和31°C相比，暴露于35°C的鱼的血糖水平显著升高。在暴露7天和14天时，与27°C和31°C相比，暴露于35°C的鱼血液中的Hb和RBC含量降低，但WBC显著增加。因此，与27°C和31°C相比，发现暴露于35°C的鱼血液中的成红细胞（Ebs）、红细胞核异常（ENA）和红细胞细胞异常（ECA）的频率增加。在最高温度（35°C）下，中性粒细胞显著增加，淋巴细胞减少。在研究期间，随着温度升高，溶解氧（DO）降低，但游离CO显著增加。本研究表明，鲤鱼更适应27°C和31°C的环境温度，而较高温度35°C可能对该鱼类有压力。
