Pot Caroline, Simonen Marjo, Weinmann Oliver, Schnell Lisa, Christ Franziska, Stoeckle Sascha, Berger Philipp, Rülicke Thomas, Suter Ueli, Schwab Martin E
Brain Research Institute, University of Zurich, and Department of Biology, Swiss Federal Institute of Technology Zurich, CH-8057 Zurich, Switzerland.
J Cell Biol. 2002 Oct 14;159(1):29-35. doi: 10.1083/jcb.200206068.
Injured axons in mammalian peripheral nerves often regenerate successfully over long distances, in contrast to axons in the brain and spinal cord (CNS). Neurite growth-inhibitory proteins, including the recently cloned membrane protein Nogo-A, are enriched in the CNS, in particular in myelin. Nogo-A is not detectable in peripheral nerve myelin. Using regulated transgenic expression of Nogo-A in peripheral nerve Schwann cells, we show that axonal regeneration and functional recovery are impaired after a sciatic nerve crush. Nogo-A thus overrides the growth-permissive and -promoting effects of the lesioned peripheral nerve, demonstrating its in vivo potency as an inhibitor of axonal regeneration.
与脑和脊髓（中枢神经系统）中的轴突不同，哺乳动物外周神经中受损的轴突通常能成功地进行长距离再生。神经突生长抑制蛋白，包括最近克隆出的膜蛋白Nogo - A，在中枢神经系统中含量丰富，尤其是在髓磷脂中。在外周神经髓磷脂中检测不到Nogo - A。通过在外周神经施万细胞中调控Nogo - A的转基因表达，我们发现坐骨神经挤压伤后轴突再生和功能恢复受到损害。因此，Nogo - A克服了受损外周神经的生长允许和促进作用，证明了其作为轴突再生抑制剂在体内的效力。
