Yeh Kuang-Sheng, Tsai Chin-En, Chen Shih-Ping, Liao Chao-Wei
Division of Animal Medicine, Animal Technology Institute Taiwan, Chunan, Miaoli.
J Food Prot. 2002 Oct;65(10):1656-9. doi: 10.4315/0362-028x-65.10.1656.
VIDAS Salmonella (VIDAS-SLM) is an automated system that uses the enzyme-linked fluorescent assay method to detect Salmonella species. This study evaluated the efficacy of the VIDAS-SLM method in detecting Salmonella species in pork carcass sponge samples gathered from 10 slaughter plants in Taiwan. Two hundred fifty-seven pork carcass sponge samples were screened by the VIDAS-SLM method and by the culture method in parallel. While 18 sponge samples were found to test positive by both methods, the VIDAS-SLM method detected four additional positive samples for which the culture method failed to recover Salmonella. The specificity of the VIDAS-SLM method was found to be 0.98, and its sensitivity was 1.0, since no false-negative results occurred. Artificially inoculated Salmonella at concentrations as low as 5.0 x 10(0) CFU/ml was detected in the heat-inactivated sponge sample in the presence or absence of 5.0 x 10(4) CFU of Citrobacter freundii per ml. Thus, the VIDAS-SLM method is a rapid screening method and a potential alternative to the time- and labor-intensive culture method.
VIDAS沙门氏菌检测系统（VIDAS - SLM）是一种利用酶联荧光分析法检测沙门氏菌属的自动化系统。本研究评估了VIDAS - SLM方法在检测从台湾10家屠宰场采集的猪胴体海绵样本中沙门氏菌属的效果。采用VIDAS - SLM方法和培养法对257份猪胴体海绵样本进行了平行筛查。两种方法均检测出18份海绵样本呈阳性，而VIDAS - SLM方法还检测出另外4份阳性样本，培养法未能从这些样本中分离出沙门氏菌。由于未出现假阴性结果，VIDAS - SLM方法的特异性为0.98，敏感性为1.0。在每毫升含有或不含有5.0×10⁴CFU弗氏柠檬酸杆菌的热灭活海绵样本中，均可检测到低至5.0×10⁰CFU/ml浓度的人工接种沙门氏菌。因此，VIDAS - SLM方法是一种快速筛查方法，有望替代耗时费力的培养法。
