用于食品中沙门氏菌检测的Vitek免疫诊断分析系统（VIDAS）评估

Evaluation of the Vitek Immunodiagnostic Assay System (VIDAS) for the detection of Salmonella in foods.

作者信息

Blackburn C W, Curtis L M, Humpheson L, Petitt S B

机构信息

Department of Food Microbiology, Leatherhead Food Research Association, Surrey, UK.

出版信息

Lett Appl Microbiol. 1994 Jul;19(1):32-6. doi: 10.1111/j.1472-765x.1994.tb00897.x.

DOI:10.1111/j.1472-765x.1994.tb00897.x

PMID:7765217

Abstract

A Salmonella Assay using the Vitek Immunodiagnostic Assay System (VIDAS) was compared with a conventional cultural method (CCM) for the detection of salmonellas in 141 samples of artificially and naturally contaminated foods. There was an overall agreement of 92.9% between the methods. The productivity of the VIDAS Salmonella Assay (VSA) was not improved using an alternative enrichment protocol for the detection of Salmonella in 12 raw meat samples. The sensitivity and specificity of the VSA was assessed using pure cultures of salmonellas and non-salmonellas. The detection limit was 1.8 x 10(6) salmonellas ml-1 in M-broth and some Citrobacter freundii strains gave false-positive results. Using an immunomagnetic separation (IMS) technique and an abbreviated cultural enrichment, the VSA results could be obtained a day earlier than the standard VSA method.

摘要

使用Vitek免疫诊断分析系统（VIDAS）的沙门氏菌检测方法与传统培养方法（CCM）在141份人工和天然污染食品样本中检测沙门氏菌的情况进行了比较。两种方法的总体一致性为92.9%。对于12份生肉样本中沙门氏菌的检测，使用替代增菌方案并未提高VIDAS沙门氏菌检测法（VSA）的效率。使用沙门氏菌和非沙门氏菌的纯培养物评估了VSA的灵敏度和特异性。在M肉汤中检测限为1.8×10⁶个沙门氏菌/毫升，一些弗氏柠檬酸杆菌菌株给出了假阳性结果。使用免疫磁珠分离（IMS）技术和简化的培养富集方法，可比标准VSA方法提前一天获得VSA结果。