Sommerhäuser Jürgen, Failing Klaus
Landeslabor Brandenburg, Laborbereich Potsdam.
Berl Munch Tierarztl Wochenschr. 2006 Jan-Feb;119(1-2):22-7.
The VIDAS Salmonella Test (VST) is an enzyme-linked fluorescent immunoassay for the detection of Salmonella-antigens. The suitability of VST for the detection of Salmonella in faecal, tissue, and feed samples was evaluated by the comparison with routine culture methods. From 312 naturally contaminated samples 17 were classified as Salmonella positive by routine methods and 28 by VST. Salmonella were isolated from 15 VST positive samples by the routine method and from eight samples only by an extended culture method. Five positive VST results could not be proved by culture. Two samples were classified as positive by the routine method and as false-negative by VST. The sensitivity varied between 88% and 100% and the specifity between 92% and 100%, depending on the kind of sample. Matrix or serovar specific factors resulting in a false VST result could not be determined. The performance of VST was easy and did not require special experiences. Mostly, samples with Salmonella negative results were faster detected than by culture methods. VST is suitable for the detection of Salmonella in the studied kind of samples especially as a screening method.
VIDAS 沙门氏菌检测（VST）是一种用于检测沙门氏菌抗原的酶联荧光免疫分析方法。通过与常规培养方法比较，评估了 VST 在粪便、组织和饲料样本中检测沙门氏菌的适用性。在 312 份自然污染样本中，常规方法将 17 份判定为沙门氏菌阳性，VST 将 28 份判定为阳性。通过常规方法从 15 份 VST 阳性样本中分离出沙门氏菌，仅通过延长培养方法从 8 份样本中分离出沙门氏菌。5 份 VST 阳性结果无法通过培养证实。2 份样本常规方法判定为阳性，VST 判定为假阴性。根据样本类型不同，灵敏度在 88%至 100%之间，特异性在 92%至 100%之间。无法确定导致 VST 结果出现假阳性的基质或血清型特异性因素。VST 的操作简便，不需要特殊经验。大多数情况下，沙门氏菌检测结果为阴性的样本通过 VST 检测比通过培养方法检测更快。VST 适用于在所研究的样本类型中检测沙门氏菌，尤其作为一种筛查方法。
