Nicollier M, Gibey R, Colette C, Henry J C
Laboratoire de Biochimie, U.E.R. des Sciences Biologiques et Médicales, 4, Place St Jacques, 25000-Besancon (France).
Clin Chim Acta. 1975 Apr 2;60(1):15-24. doi: 10.1016/0009-8981(75)90175-8.
The nature and origin of soluble proteins from human amniotic fluid have been investigated by gel filtration chromatography on Sepharose 6B and ion-exchange chromatography on Ecteola-cellulose. Each fraction has been studied by immunoelectrophoresis. Specific antisera against serum proteins, amniotic fluid proteins, fetal proteins and salivary proteins have been used. These various antisera showed that the amniotic fluid contains maternal proteins, but also more specific proteins from fetal origin such as beta2-microglobulin, urinary mucopolysaccharides, salivary proteins and carcinoembryonic antigen. These results not only confirm that amniotic proteins are essentially from maternal origin, but prove the important fetal contribution and emphasize the importance of the fetoplacental unit in the monitoring of high risk pregnancies.
通过在琼脂糖6B上进行凝胶过滤色谱法以及在ECTEOLA-纤维素上进行离子交换色谱法，对人羊水可溶性蛋白质的性质和来源进行了研究。通过免疫电泳对每个组分进行了研究。使用了针对血清蛋白、羊水蛋白、胎儿蛋白和唾液蛋白的特异性抗血清。这些不同的抗血清表明，羊水不仅含有母体蛋白，还含有更多源自胎儿的特异性蛋白，如β2-微球蛋白、尿粘多糖、唾液蛋白和癌胚抗原。这些结果不仅证实羊水蛋白基本上源自母体，还证明了胎儿的重要贡献，并强调了胎盘单位在高危妊娠监测中的重要性。
