Rubinoff Daniel, Sperling Felix A H
Division of Insect Biology, University of California, 201 Wellman Hall, Berkeley, CA 94720, USA.
Mol Phylogenet Evol. 2002 Oct;25(1):70-86. doi: 10.1016/s1055-7903(02)00213-0.
We present a molecular phylogeny for the genus Hemileuca (Saturniidae), based on 624 bp of mitochondrial cytochrome oxidase I (COI) and 932 bp of the nuclear gene elongation factor 1 alpha (EF1alpha). Combined analysis of both gene sequences increased resolution and supported most of the phylogenetic relationships suggested by separate analysis of each gene. However, a maximum parsimony (MP) model for just COI sequence from one sample of most taxa produced a phylogeny incongruent with EF1alpha and combined dataset analyses under either MP or ML models. Time of year and time of day during which adult moths fly corresponded strongly with the phylogeny. Although most Hemileuca are diurnal, ancestral Hemileuca probably were nocturnal, fall-flying insects. The two-gene molecular phylogeny suggests that wing morphology is frequently homoplastic. There was no correlation between the primary larval hostplants and phylogenetic placement of taxa. No phylogenetic pattern of specialization was evident for single hostplant families across the genus. Our results suggest that phenological behavioral characters may be more conserved than the wing morphology characters that are more commonly used to infer phylogenetic relationships in Lepidoptera. Inclusion of a molecular component in the re-evaluation of systematic data is likely to alter prior assumptions of phylogenetic relationships in groups where such potentially homoplastic characters have been used.
我们基于624个碱基对的线粒体细胞色素氧化酶I（COI）和932个碱基对的核基因延伸因子1α（EF1α），呈现了Hemileuca属（大蚕蛾科）的分子系统发育。对两个基因序列的联合分析提高了分辨率，并支持了每个基因单独分析所暗示的大多数系统发育关系。然而，对大多数分类单元的一个样本仅COI序列的最大简约（MP）模型产生的系统发育与EF1α以及在MP或ML模型下的联合数据集分析不一致。成年蛾飞行的年份时间和一天中的时间与系统发育密切相关。虽然大多数Hemileuca是日间活动的，但Hemileuca的祖先可能是夜间活动、秋季飞行的昆虫。双基因分子系统发育表明翅形态常常是同塑性的。主要幼虫寄主植物与分类单元的系统发育位置之间没有相关性。整个属中单一寄主植物科没有明显的特化系统发育模式。我们的结果表明，物候行为特征可能比鳞翅目更常用于推断系统发育关系的翅形态特征更保守。在系统数据的重新评估中纳入分子成分可能会改变在使用了这种潜在同塑性特征的类群中系统发育关系的先前假设。
