Jamal Wafaa Y, Mokaddas Eiman M, Verghese Tina L, Rotimi V O
Department of Microbiology, Faculty of Medicine, Kuwait University and Mubarak Al-Kabeer Teaching Hospital, P.O. Box 24923, 13110, Safat, Kuwait.
Int J Antimicrob Agents. 2002 Oct;20(4):270-4. doi: 10.1016/s0924-8579(02)00180-2.
A total of 73 clinical isolates of Clostridium difficile isolated from stool/rectal swabs of patients admitted to the intensive care units at Mubarak Hospital, Ibn Sina Hospital Burn unit and Haematology wards at the Kuwait Cancer Control Centre, were investigated for their susceptibility to 15 antibiotics using the Etest. Amoxycillin-clavulanic acid, ampicillin, meropenem, metronidazole, penicillin, piperacillin, piperacillin/tazobactam, teicoplanin and vancomycin had excellent activities with MIC(90)s of 0.38, 0.5, 1, 0.19, 1.5, 2, 3, 0.25 and 0.75 mg/l, respectively. Of the 73 C. difficile isolates, 86% were resistant to imipenem (MIC(90) >32 mg/l) and almost 97% were resistant to trovafloxacin (MIC(90)>256 mg/l). Forty eight percent of the isolates were resistant to clindamycin. A total of 18 isolates were highly clindamycin-resistant with an MIC of >256 mg/l; 10 of these were toxin producers. Multiple antibiotic resistance (two or more antibiotics) was noted in 63 isolates. These were more common among the toxigenic strains than the non-toxigenic strains by a ratio of 2.5:1.
从穆巴拉克医院重症监护病房、伊本·西那医院烧伤科以及科威特癌症控制中心血液科病房的患者粪便/直肠拭子中分离出73株艰难梭菌临床分离株，使用Etest法检测它们对15种抗生素的敏感性。阿莫西林-克拉维酸、氨苄西林、美罗培南、甲硝唑、青霉素、哌拉西林、哌拉西林/他唑巴坦、替考拉宁和万古霉素具有优异的活性，其MIC（90）分别为0.38、0.5、1、0.19、1.5、2、3、0.25和0.75mg/l。在73株艰难梭菌分离株中，86%对亚胺培南耐药（MIC（90）>32mg/l），近97%对曲伐沙星耐药（MIC（90）>256mg/l）。48%的分离株对克林霉素耐药。共有18株分离株对克林霉素高度耐药，MIC>256mg/l；其中10株产生毒素。63株分离株存在多重抗生素耐药（对两种或更多种抗生素耐药）。这些在产毒菌株中比非产毒菌株更常见，比例为2.5:1。
