Zeitoun Moustafa Mohamed, El-Dawas Abdulrahman O, Ateah Mohamed A, El-Deen Mohamed Ahmed Shehab
Department of Animal Production and Breeding, College of Agriculture and Veterinary Medicine, Qassim University 51452, Saudi Arabia.
Department of Animal and Fish Production, Faculty of Agriculture, El-Shatby, Alexandria University, Alexandria 21545, Egypt.
Vet World. 2019 Apr;13(4):633-641. doi: 10.14202/vetworld.2020.633-641. Epub 2020 Apr 8.
The aim of this study was to investigate the effectiveness of using recombinant human follicle-stimulating hormone (FSH) compared with pituitary-derived porcine FSH given as one dose or multiple doses on the neonatal traits, follicular dynamics, and maternal blood biochemical constituents in ewes.
A 3×2 factorial arrangement was designed utilizing 60 adults ewes to test the effects of using two sources of FSH ( vs) in addition to control, either given as a single total dose or six descending doses to provoke twinning. Six treatments (T) were tested (n=10 ewes/T). C1 and C6 served as control ewes given saline as one dose and six doses, respectively; H1 and H6 ewes were given human FSH as one and six doses; and P1 and P6 ewes were given porcine FSH similar to the above treatments. Saline and/or FSH administration were administered at days 8, 9, and 10 of the 10-day controlled internal drug release (CIDR) implant. At CIDR removal, fertile rams were used for natural mating. Blood samples for the assessment of serum metabolites were collected.
Twinning increased in FSH-treated ewes than control. However, giving FSH of either source as a single dose resulted in a higher incidence of stillbirths. Pregnancy rates were 30, 40, 50, 60, 70, and 80% in C1, C6, P1, P6, H1, and H6, respectively. Respective percent of ewes delivering twins/multiple birth was 0, 0, 80, 66.7, 71.4, and 87.5%. FSH of human source was more efficient for folliculogenesis than porcine FSH. Administration of FSH increased blood cholesterol, decreased high-density lipoprotein; however, low-density lipoprotein levels were not different than control. Moreover, an interaction (p<0.05) exists between source and type of FSH administration on blood glucose. Six doses of FSH elevated blood protein. Blood albumin decreased by porcine-FSH but not affected by human-FSH. Blood globulins were not different due to source of FSH, whereas giving FSH as six doses increased globulins than in single-dose protocol. Contrariwise, an interaction was found between source and type of FSH administration on elevating the activity of alanine aminotransferase and reducing the activity of aspartate aminotransferase.
Administration of human FSH at 180 IU in six descending doses resulted in the best neonatal outcomes and maternal health in ewes.
本研究旨在调查与垂体来源的猪促卵泡素（FSH）相比，单剂量或多剂量使用重组人促卵泡素对母羊新生羔羊性状、卵泡动态及母羊血液生化成分的影响。
采用3×2析因设计，利用60只成年母羊来测试两种促卵泡素来源（对比）的效果，此外还设置了对照组，将促卵泡素以单次总剂量或六次递减剂量给药以诱导双胎。测试了六种处理方式（T）（每组n = 10只母羊）。C1和C6作为对照组母羊，分别给予单剂量和六剂量的生理盐水；H1和H6组母羊分别给予单剂量和六剂量的人促卵泡素；P1和P6组母羊给予与上述处理类似剂量的猪促卵泡素。在10天的控制内部药物释放（CIDR）植入的第8、9和10天给予生理盐水和/或促卵泡素。取出CIDR后，使用可育公羊进行自然交配。采集血液样本以评估血清代谢产物。
与对照组相比，促卵泡素处理的母羊双胎率增加。然而，单剂量给予任何一种来源的促卵泡素都会导致死胎发生率更高。C1、C6、P1、P6、H1和H6组的妊娠率分别为30%、40%、50%、60%、70%和80%。产双胎/多胎的母羊各自所占百分比分别为0、0、80%、66.7%、71.4%和87.5%。人源促卵泡素在卵泡生成方面比猪源促卵泡素更有效。促卵泡素的给药会使血液胆固醇升高，高密度脂蛋白降低；然而，低密度脂蛋白水平与对照组无差异。此外，促卵泡素来源和给药方式对血糖存在交互作用（p<0.05）。六剂量的促卵泡素会使血液蛋白升高。猪源促卵泡素会使血液白蛋白降低，但人源促卵泡素对其无影响。由于促卵泡素来源不同，血液球蛋白无差异，而与单剂量给药方案相比，六剂量给予促卵泡素会使球蛋白升高。相反，在提高丙氨酸转氨酶活性和降低天冬氨酸转氨酶活性方面，发现促卵泡素来源和给药方式之间存在交互作用。
以六次递减剂量给予180国际单位的人促卵泡素可使母羊获得最佳的新生羔羊结局和母羊健康状况。
