Alijani A, Niasari-Naslaji A, Sayyah N, Baninajar M, Ganjkhanlou M
DVSc Student in Veterinary Obstetrics and Gynecology, Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Iran J Vet Res. 2024;25(1):3-7. doi: 10.22099/IJVR.2024.49344.7239.
It is desirable in estrus synchronization in sheep to avoid intravaginal devices and to shorten the program from 14 to 6 days. Moreover, replacement of eCG with safe, cheap, and efficient gonadotropin is in worldwide demand.
This study investigates the possibility of replacing eCG with human recombinant FSH (hrFSH) and CIDR with progesterone injections for estrous synchronization in ewes.
Assaf and Lacaune ewes (n=170) were divided into two groups and synchronized with either progesterone injections for 6 days or CIDR for 14 days. Ewes assigned in the injection group, received progesterone (37.5 mg; SC) and GnRH analogue (7.5 µg Alarelin acetate; IM) on day 0 of the experiment. On days 3 and 6, ewes received 25 and 12.5 mg progesterone (SC), respectively. On day 6, ewes in both groups received prostaglandin Fα (250 µg Cloprostenol; IM), and were divided into two subgroups to receive either hrFSH (75 IU Follitropin alfa; SC) or eCG (400 IU; IM). On day 7, fertile rams were introduced to ewes for 21 days. Data were analyzed using GLM and Glimmix.
There was no difference in the respective lambing rates, prolificacy, and fecundity between CIDR (71.1, 1.63, and 1.16%) and injections (66.7, 1.55, and 1.03%); between eCG (71.4, 1.60, and 1.14%), and hrFSH (66.3, 1.58, and 1.05%, P>0.05).
In conclusion, 6-day progesterone injection-based protocol produced similar results to 14-day CIDR program and hrFSH could be an effective alternative for eCG during estrus synchronization in ewes.
在绵羊发情同步化过程中，希望避免使用阴道内装置，并将程序从14天缩短至6天。此外，用安全、廉价且高效的促性腺激素替代eCG是全球的需求。
本研究探讨用重组人促卵泡素（hrFSH）替代eCG以及用孕酮注射替代阴道孕酮释放装置（CIDR）进行母羊发情同步化的可能性。
将170只阿萨夫羊和拉克瑙羊分为两组，分别用孕酮注射6天或CIDR处理14天进行发情同步化。注射组的母羊在实验第0天接受孕酮（37.5mg；皮下注射）和促性腺激素释放激素类似物（7.5μg醋酸阿拉瑞林；肌肉注射）。在第3天和第6天，母羊分别接受25mg和12.5mg孕酮（皮下注射）。在第6天，两组母羊均接受前列腺素Fα（250μg氯前列醇；肌肉注射），并分为两个亚组，分别接受hrFSH（75IU注射用重组人促卵泡激素α；皮下注射）或eCG（400IU；肌肉注射）。在第7天，将可育公羊放入母羊群中21天。数据采用广义线性模型（GLM）和广义线性混合模型（Glimmix）进行分析。
CIDR组（71.1%、1.63和1.16%）和注射组（66.7%、1.55和1.03%）的产羔率、产仔数和繁殖力之间无差异；eCG组（71.4%、1.60和1.14%）和hrFSH组（66.3%、1.58和1.05%，P>0.05）之间也无差异。
总之，基于6天孕酮注射的方案与14天CIDR方案产生的结果相似，并且在母羊发情同步化过程中，hrFSH可以作为eCG的有效替代品。
