Ito A
Parasitology. 1975 Dec;71(3):465-73. doi: 10.1017/s0031182000047223.
Oncospheral agglutination given by sera immunized with Hymenolepis nana eggs is described as a new way of assessing H. nana infection. All sera of mice which possessed acquired protective immunity against reinfection by H. nana eggs had the potency to induce oncospheral agglutination in vitro. Only oncospheres, which had been hatched, agglutinated, no agglutination occurred in sera from uninfected mice. Oncospheral agglutination was carried out by mixing 0-1 ml of serial two-fold dilutions of serum and 0-1 ml of Hanks' balanced salt solution containing about 600 hatched oncospheres. Titre of agglutinins was indicated as a reciprocal of the final dilution capable of giving agglutination clusters made of three or more oncospheres. Agglutinins developed within 14 days after a primary infection with 500 shell-free eggs. There was no rapid increase of agglutinins within 4 days following a secondary infection. The titre increase coincided with the increase in dosages of eggs. Agglutinins were thought to be immunoglobulins, because the potency of the serum to agglutinate oncospheres was extinguished after absorption of globulins with rabbit anti-mouse globulin serum. Agglutinins were produced in rabbits by intravenous injections of shell-free eggs. The titres of the rabbit sera were much higher than those of mouse sera.
用微小膜壳绦虫虫卵免疫的血清所产生的六钩蚴凝集反应被描述为评估微小膜壳绦虫感染的一种新方法。所有对微小膜壳绦虫虫卵再感染具有获得性保护性免疫的小鼠血清，在体外均有诱导六钩蚴凝集的能力。只有已孵化的六钩蚴发生凝集，未感染小鼠的血清中不发生凝集。六钩蚴凝集反应是通过将0.1ml血清的系列两倍稀释液与0.1ml含有约600个已孵化六钩蚴的汉克斯平衡盐溶液混合进行的。凝集素效价表示为能够产生由三个或更多六钩蚴组成的凝集簇的最终稀释倍数的倒数。在用500个无壳虫卵初次感染后14天内产生凝集素。二次感染后4天内凝集素无快速增加。效价增加与虫卵剂量增加一致。凝集素被认为是免疫球蛋白，因为用兔抗小鼠球蛋白血清吸收球蛋白后，血清凝集六钩蚴的能力消失。通过静脉注射无壳虫卵在兔体内产生凝集素。兔血清的效价比小鼠血清的效价高得多。
