钠离子介导的亚基间桥联作为钠离子转运型F1F0 ATP合酶c环异常稳定性的一种解释。
Intersubunit bridging by Na+ ions as a rationale for the unusual stability of the c-rings of Na+-translocating F1F0 ATP synthases.
作者信息
Meier Thomas, Dimroth Peter
机构信息
Institut für Mikrobiologie, Eidgenössische Technische Hochschule, ETH-Zentrum, CH-8092 Zürich, Switzerland.
出版信息
EMBO Rep. 2002 Nov;3(11):1094-8. doi: 10.1093/embo-reports/kvf216. Epub 2002 Oct 22.
Abstract
The oligomeric c-rings of Na+-translocating F1F0 ATP synthases exhibit unusual stability, resisting even boiling in SDS. Here, we show that the molecular basis for this remarkable property is intersubunit crossbridging by Na+ or Li+ ions. The heat stability of c11 was dependent on the presence of Na+ or Li+ ions. For equal stability, 10 times higher Li+ than Na+ concentrations were required, reflecting the 10 times lower binding affinity for Li+ than for Na+. In a recent structural model of c11, the Na+ or Li+ binding ligands are located on neighboring c-subunits, which thus become crossbridged by the binding of either alkali ion with a concomitant increase in the stability of the ring. Site-directed mutagenesis strengthens the essential role of glutamate 65 in the crossbridging of the subunits and also corroborates the proposed stabilizing effect of an ion bridge including aspartate 2.
摘要
Na⁺转运型F₁F₀ ATP合酶的寡聚c环表现出异常的稳定性，甚至在SDS中煮沸也能抵抗。在这里，我们表明这种显著特性的分子基础是通过Na⁺或Li⁺离子进行亚基间交联。c₁₁的热稳定性取决于Na⁺或Li⁺离子的存在。为了达到相同的稳定性，所需的Li⁺浓度比Na⁺浓度高10倍，这反映出Li⁺的结合亲和力比Na⁺低10倍。在最近的c₁₁结构模型中，Na⁺或Li⁺结合配体位于相邻的c亚基上，因此通过任一碱金属离子的结合使这些亚基交联，同时环的稳定性增加。定点诱变强化了谷氨酸65在亚基交联中的重要作用，也证实了包括天冬氨酸2在内的离子桥的拟稳定作用。
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