Decostere A, Henckaerts K, Ducatelle R, Haesebrouck F
Laboratory of Veterinary Bacteriology and Mycology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B 9820 Merelbeke, Belgium.
Lab Anim. 2002 Oct;36(4):396-402. doi: 10.1258/002367702320389053.
This study describes the development of a trout gill perfusion model, consisting of an excised branchial arch from rainbow trout (Oncorhynchus mykiss L.), perfused via the afferent branchial artery and suspended in a circular organ chamber filled with Ringer solution. Different perfusion fluids were tested: Ringer, Cortland, Ringer + procaine, Ringer + adrenalin, Cortland + procaine, Cortland + adrenalin and Cortland + dextran 1%. The latter perfusion fluid proved to be satisfactory, maintaining the gill tissue in a healthy condition outside the body of the fish for at least 180 min. Using this model, the interaction of damaging agents with the trout gill tissue may be studied under carefully controlled conditions. The trout gill perfusion model leads effectively to a reduction in the number of experimental animals to be used and also involves an elimination of pain and/or suffering, which is as good as complete.
本研究描述了一种虹鳟鱼鳃灌注模型的建立，该模型由虹鳟（Oncorhynchus mykiss L.）的离体鳃弓组成，通过鳃传入动脉进行灌注，并悬浮于充满林格液的圆形器官腔室中。测试了不同的灌注液：林格液、科特兰液、林格液+普鲁卡因、林格液+肾上腺素、科特兰液+普鲁卡因、科特兰液+肾上腺素和科特兰液+1%右旋糖酐。结果证明，后一种灌注液效果良好，可使鳃组织在鱼体外保持健康状态至少180分钟。利用该模型，可以在严格控制的条件下研究损伤因子与虹鳟鱼鳃组织的相互作用。虹鳟鱼鳃灌注模型有效地减少了实验动物的使用数量，并且还消除了疼痛和/或痛苦，几乎达到了完全消除的程度。
