United States Department of Agriculture, Agricultural Research Service, Natural Products Utilization Research Unit, National Center for Natural Products Research, Post Office Box 1848, University, MS 38677, USA.
Chemistry of Natural Compounds Department, Pharmaceutical and Drug Industries Division, National Research Centre, Dokki, Giza 12622, Egypt.
Molecules. 2018 Oct 25;23(11):2761. doi: 10.3390/molecules23112761.
Enteric septicemia of catfish, columnaris disease and streptococcosis, caused by , and , respectively, are the most common bacterial diseases of economic significance to the pond-raised channel catfish industry. Certain management practices are used by catfish farmers to prevent large financial losses from these diseases such as the use of commercial antibiotics. In order to discover environmentally benign alternatives, using a rapid bioassay, we evaluated a crude extract from the roots of muscadine against these fish pathogenic bacteria and determined that the extract was most active against . Subsequently, several isolated compounds from the root extract were isolated. Among these isolated compounds, (+)-hopeaphenol () and (+)-vitisin A () were found to be the most active (bacteriostatic activity only) against , with 24-h 50% inhibition concentrations of 4.0 ± 0.7 and 7.7 ± 0.6 mg/L, respectively, and minimum inhibitory concentrations of 9.1 ± 0 mg/L for each compound which were approximately 25X less active than the drug control florfenicol. Efficacy testing of and is necessary to further evaluate the potential for these compounds to be used as antibacterial agents for managing columnaris disease.
斑点叉尾鮰肠败血症、柱状病和链球菌病分别由、和引起，是池塘养殖斑点叉尾鮰产业中最常见的具有经济重要性的细菌性疾病。为了防止这些疾病（如商业抗生素的使用）造成巨大的经济损失，鲶鱼养殖户采用了某些管理措施。为了寻找环保的替代品，我们使用快速生物测定法评估了葡萄根的粗提取物对这些鱼类病原菌的作用，并确定该提取物对最具活性。随后，从根提取物中分离出几种分离化合物。在这些分离出的化合物中，（+）-hopaphenol（）和（+）-vitisin A（）被发现对最具活性（仅抑菌活性），对的 24 小时 50%抑制浓度分别为 4.0±0.7 和 7.7±0.6mg/L，每个化合物的最小抑菌浓度为 9.1±0mg/L，分别比药物对照氟苯尼考低约 25 倍。需要对和进行疗效测试，以进一步评估这些化合物作为管理柱状病的抗菌剂的潜力。
