Mutschler T, Kieser B, Frank R, Gauglitz G
Institute of Theoretical and Physical Chemistry, Eberhard-Karls-University, Auf der Morgenstelle 8, 72076 Tübingen, Germany.
Anal Bioanal Chem. 2002 Oct;374(4):658-64. doi: 10.1007/s00216-002-1488-3. Epub 2002 Sep 13.
The characterization of sensitive layers is the prerequisite for the optimization of chemical and biochemical sensors. The application of SE (Spectral Ellipsometry) and SPR (Surface Plasmon Resonance) as methods of characterization of such sensitive layers is discussed. In combination with infrared spectroscopy, the properties of polymer networks, micro-porous polymers, liquid crystals, and biomimetic polymers can be examined regarding their applicability for optical sensing. Apart from the basic principles regarding the characterization approaches, applications in the area of environmental sensing, optimization of hydrogel layers for antigen/antibody interaction, and discrimination of analytes in homologous series of alcohols are discussed. The effects of analytes on the phase transition in combination with disordering of liquid crystals are given.
敏感层的表征是优化化学和生化传感器的前提。本文讨论了将光谱椭偏仪（SE）和表面等离子体共振（SPR）作为此类敏感层表征方法的应用。结合红外光谱，可以研究聚合物网络、微孔聚合物、液晶和仿生聚合物的性质，以考察它们在光学传感方面的适用性。除了关于表征方法的基本原理外，还讨论了在环境传感领域的应用、用于抗原/抗体相互作用的水凝胶层的优化以及醇类同系物中分析物的鉴别。文中给出了分析物对液晶相变及无序化的影响。
