Henry Olivier Y F, Cullen David C, Piletsky Sergey A
Institute of Bioscience and Technology, Cranfield University, Silsoe, Bedfordshire MK45 4DT, UK.
Anal Bioanal Chem. 2005 Jun;382(4):947-56. doi: 10.1007/s00216-005-3255-8. Epub 2005 Jun 7.
This article reviews the progress and developments achieved in the past five years (2000-2005) in the application of optical analytical techniques to the evaluation of molecularly imprinted polymer (MIP) characteristics. The MIP binding efficiency, recognition processes and selectivity have been intensively studied by optical means due to the general high sensitivity and simplicity of the utilisation of optical techniques. In addition, recent progress in the covalent linkage of MIPs to optical transducers has allowed for the realisation of highly efficient and robust optical MIP-based molecular recognition sensors. The review provides insight into the various approaches to the optical interrogation of MIPs, and is organised according to the type of optical technique employed (fluorescence, UV/Vis and infrared spectroscopy, surface plasmon resonance, chemiluminescence, refractive interference spectroscopy and Raman scattering) and the detailed strategies applied. The review also covers the recent progress achieved in the area of optical sensors based on MIPs.
本文综述了过去五年（2000 - 2005年）光学分析技术在评估分子印迹聚合物（MIP）特性方面取得的进展。由于光学技术通常具有高灵敏度和使用简便性，因此通过光学手段对MIP的结合效率、识别过程和选择性进行了深入研究。此外，MIP与光学换能器共价连接的最新进展使得基于MIP的高效且稳健的光学分子识别传感器得以实现。该综述深入探讨了对MIP进行光学检测的各种方法，并根据所采用的光学技术类型（荧光、紫外/可见和红外光谱、表面等离子体共振、化学发光、折射干涉光谱和拉曼散射）以及应用的详细策略进行组织。该综述还涵盖了基于MIP的光学传感器领域的最新进展。
